| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,155,146:1 | +A | 23.4% | coding (783/870 nt) | prkB ← | predicted phosphoribulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,155,146 | 1 | . | A | 23.4% | 105.9 / 45.5 | 47 | coding (783/870 nt) | prkB | predicted phosphoribulokinase |
| Reads supporting (aligned to +/- strand): ref base . (9/27); new base A (5/6); total (14/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01 | |||||||||||
AATCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGGGAAAT > W3110S.gb/4155084‑4155207 | aaTCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACg < 1:2200207/70‑1 (MQ=255)aaTCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACg < 1:1781522/70‑1 (MQ=255)aaTCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACg < 1:1305306/70‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTAtt < 1:2611828/71‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTAtt < 1:1147243/71‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTAtt < 1:3115468/71‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTAtt < 1:3149094/71‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTAtt < 1:1070145/70‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTAtt < 1:280912/70‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTAtt < 1:3050156/70‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTAtt < 1:1237777/70‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTAtt < 1:483325/70‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTAtt < 1:1177152/70‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTAtt < 1:800172/70‑1 (MQ=255) gTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTAtt < 1:957039/70‑1 (MQ=255) ttGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTAtt < 1:45124/70‑1 (MQ=255) ttGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTAtt < 1:218085/70‑1 (MQ=255) ttGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTAtt < 1:1946945/69‑1 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTTGGAaatt < 1:2437199/71‑2 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGTGAaatg < 1:1916034/71‑1 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatg < 1:3263087/71‑1 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatg < 1:3026114/71‑1 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatg < 1:512471/71‑1 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatg < 1:2453003/71‑1 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatg < 1:251856/71‑1 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatg < 1:1296804/71‑1 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatg < 1:1388452/71‑1 (MQ=255) cagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGt < 1:3030038/54‑1 (MQ=255) cagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGt < 1:1150544/54‑1 (MQ=255) cagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGt < 1:2756926/54‑1 (MQ=255) cagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCAGGTACC‑ACTAACGt < 1:3305102/54‑1 (MQ=255) aTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatgaat > 1:374258/1‑69 (MQ=255) aattCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTg > 1:2653473/1‑71 (MQ=255) aattCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGACTTg > 1:1262876/1‑71 (MQ=255) attCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGc < 1:199251/71‑1 (MQ=255) ttacATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTg > 1:11614/4‑70 (MQ=255) ttCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAGCGTATTGATGTGGGAAATGAATGAGCCTTg > 1:3020148/1‑70 (MQ=255) ttCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTg > 1:1205760/1‑70 (MQ=255) ttCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTg > 1:1385478/1‑70 (MQ=255) cATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACa < 1:2188058/71‑1 (MQ=255) gCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGc > 1:623210/1‑71 (MQ=255) gCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGc > 1:1073862/1‑71 (MQ=255) gCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGc > 1:3147952/1‑71 (MQ=255) gCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGc > 1:2004712/1‑71 (MQ=255) gCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGc > 1:2917868/1‑71 (MQ=255) gCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGc > 1:2305346/1‑71 (MQ=255) cccGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGGGAAAt > 1:3007594/1‑71 (MQ=255) | AATCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGGGAAAT > W3110S.gb/4155084‑4155207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |