Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 547,831:1 +G 100% pseudogene (251/261 nt)
pseudogene (251/1259 nt)		 ylbE →
ylbE →		 ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb547,8311.G100.0% 46.0 / NA 14pseudogene (251/261 nt)
pseudogene (251/1259 nt)		ylbE
ylbE		ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (7/7);  total (7/7)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCC  >  W3110S.gb/547778‑547870
                                                      |                                       
tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc                          >  1:2049313/1‑70 (MQ=255)
tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCa                         >  1:2519868/1‑71 (MQ=255)
tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCa                         >  1:2827460/1‑71 (MQ=255)
tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCa                         >  1:69793/1‑71 (MQ=255)
tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCa                         >  1:972884/1‑71 (MQ=255)
                 cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg        <  1:1170834/71‑1 (MQ=255)
                 cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg        <  1:3120310/71‑1 (MQ=255)
                  gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg        <  1:803724/70‑1 (MQ=255)
                       aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc  <  1:1134075/71‑1 (MQ=255)
                       aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc  <  1:2702936/71‑1 (MQ=255)
                       aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc  <  1:278163/71‑1 (MQ=255)
                       aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc  <  1:465062/71‑1 (MQ=255)
                                    gcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa      >  1:1640527/1‑54 (MQ=39)
                                              cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa      >  1:1313069/1‑44 (MQ=38)
                                                      |                                       
TCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCC  >  W3110S.gb/547778‑547870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑