| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,052,400 | T→C | 12.8% | Y33H (TAC→CAC) | ugpQ → | glycerophosphodiester phosphodiesterase, cytosolic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,052,400 | 0 | T | C | 12.8% | 185.0 / 17.3 | 70 | Y33H (TAC→CAC) | ugpQ | glycerophosphodiester phosphodiesterase, cytosolic |
| Reads supporting (aligned to +/- strand): ref base T (39/22); new base C (3/6); total (42/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
GTCGCTCATCGTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCTCCATG > W3110S.gb/4052331‑4052466 | gtcgCTCATCGTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAAt > 1:2192028/1‑70 (MQ=255)gtcgCTCATCGTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATa > 1:2629598/1‑71 (MQ=255)gtcgCTCATCGTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATa > 1:1823451/1‑71 (MQ=255)gtcgCTCATCGTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATa > 1:2738527/1‑71 (MQ=255)gtcgCTCATCGTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATa > 1:3344767/1‑71 (MQ=255)gtcgCTCATCGTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATa > 1:621225/1‑71 (MQ=255)gtcgCTCATCGTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATa > 1:916267/1‑71 (MQ=255) cATCGTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCa > 1:2727845/1‑71 (MQ=255) gTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCAt > 1:1244162/1‑68 (MQ=255) ggTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATc > 1:1578746/1‑69 (MQ=255) ggTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCg > 1:490154/1‑70 (MQ=255) ggTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCg > 1:649185/1‑70 (MQ=255) ggTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCg > 1:2693033/1‑70 (MQ=255) ggTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCg > 1:1987240/1‑70 (MQ=255) ggTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCg > 1:3073358/1‑70 (MQ=255) cTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAAttt > 1:2034387/1‑69 (MQ=255) cccTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGaaa < 1:2251991/71‑1 (MQ=255) cccTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGaaa < 1:217098/71‑1 (MQ=255) cccTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGaaa < 1:945281/71‑1 (MQ=255) cccTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGaaa < 1:1809022/71‑1 (MQ=255) cccTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGaaa < 1:55290/71‑1 (MQ=255) ccTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAg < 1:3109061/71‑1 (MQ=255) ccTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAg < 1:3341492/71‑1 (MQ=255) ccTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAg < 1:1332539/71‑1 (MQ=255) ggCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATg > 1:2991618/1‑71 (MQ=255) ggCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATg > 1:3199877/1‑71 (MQ=255) ggCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATg > 1:3300084/1‑71 (MQ=255) ggCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATg > 1:571190/1‑71 (MQ=255) ggCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATg > 1:2657385/1‑71 (MQ=255) ggCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATg > 1:1167939/1‑71 (MQ=255) ggCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATg > 1:1524508/1‑71 (MQ=255) tCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGt < 1:1057383/55‑1 (MQ=255) tCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGt < 1:1392096/55‑1 (MQ=255) tCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGt < 1:1801553/55‑1 (MQ=255) aaTCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTa > 1:2556222/1‑55 (MQ=255) aaTCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTa > 1:2584746/1‑55 (MQ=255) aaTCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTa > 1:519610/1‑55 (MQ=255) aaTCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTa > 1:440160/1‑55 (MQ=255) aaTCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTa > 1:1841069/1‑55 (MQ=255) aaTCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTa > 1:2808964/1‑55 (MQ=255) aaTCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTa > 1:1509299/1‑55 (MQ=255) aaTCGACGTCGGGGCAAAACACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCgag < 1:1108223/70‑1 (MQ=255) aaTCGACGTCGGGGCAAAACACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCgag < 1:2391665/70‑1 (MQ=255) aaTCGACGTCGGGGCAAAACACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCgag < 1:1736038/70‑1 (MQ=255) aaTCGACGTCGGGGCAAAACACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCgag < 1:2974640/70‑1 (MQ=255) aaTCGACGTCGGGGCAAAACACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCgag < 1:2608981/70‑1 (MQ=255) gACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCtt > 1:2847682/1‑71 (MQ=255) gACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCtt > 1:777462/1‑71 (MQ=255) gACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCtt > 1:3205715/1‑71 (MQ=255) gACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCtt > 1:1352110/1‑71 (MQ=255) gACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCtt > 1:1916710/1‑71 (MQ=255) tCGGGGCAAAACACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCgag < 1:1148161/62‑1 (MQ=255) ggggCAAAACACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCt > 1:1526429/1‑71 (MQ=255) ggggCAAAACACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCt > 1:2834530/1‑71 (MQ=255) ggggCAAAACACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCt > 1:143808/1‑71 (MQ=255) aaaaTACGGTCATAAGATGATCGAATTTGACTCGAAGt < 1:52831/38‑1 (MQ=255) aaaaTACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCTCCATg < 1:2482691/71‑1 (MQ=255) aaaaTACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCTCCATg < 1:739957/71‑1 (MQ=255) aaaaTACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCTCCATg < 1:1357352/71‑1 (MQ=255) aaaaTACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCTCCATg < 1:405306/71‑1 (MQ=255) aaaaTACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCTCCATg < 1:3161581/71‑1 (MQ=255) aaaaTACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCTCCATg < 1:2867531/71‑1 (MQ=255) aaaTACGGTCATAAGATGATCGAATTTGACGCGAAGt < 1:2390176/37‑1 (MQ=255) aaaTACGGTCATAAGATGATCGAATTTGACGCGAAGt < 1:1460102/37‑1 (MQ=255) aaaTACGGTCATAAGATGATCGAATTTGACGCGAAGt < 1:2819697/37‑1 (MQ=255) aaaTACGGTCATAAGATGATCGAATTTGACGCGAAGt < 1:897823/37‑1 (MQ=255) aTACGGTCATAAGATGATCGTATTTGACGCGAAGTTa > 1:2932463/1‑37 (MQ=255) aTACGGTCATAAGATGATCGATTTTGACGCGAAGTTa > 1:2030483/1‑37 (MQ=255) aTACGGTCATAAGATGATCGAATTTGACGCGAAGTTa > 1:387905/1‑37 (MQ=255) aTACGGTCATAAGATGATCGAATTTGACGCGAAGTTa > 1:1253857/1‑37 (MQ=255) aTACGGTCATAAGATGATCGAATTTGACGCGAAGTTa > 1:3298618/1‑37 (MQ=255) | GTCGCTCATCGTGGCGGCGGTAAGCTGGCCCCGGAAAACACCCTGGCGTCAATCGACGTCGGGGCAAAATACGGTCATAAGATGATCGAATTTGACGCGAAGTTATCGAAAGATGGCGAGATCTTCCTGCTCCATG > W3110S.gb/4052331‑4052466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |