| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,086,494 | C→T | 8.6% | M280I (ATG→ATA) | malT ← | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,086,494 | 0 | C | T | 8.6% | 172.3 / 5.7 | 58 | M280I (ATG→ATA) | malT | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Reads supporting (aligned to +/- strand): ref base C (36/16); new base T (3/2); total (40/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
ACGCTCAATCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTATCCAA > W3110S.gb/4086425‑4086556 | aCGCTCAATCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTc < 1:1889759/70‑1 (MQ=255)aCGCTCAATCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCa > 1:891723/1‑71 (MQ=255)aCGCTCAATCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCa > 1:85575/1‑71 (MQ=255)aCGCTCAATCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCa > 1:1322211/1‑71 (MQ=255)aCGCTCAATCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCa > 1:1327087/1‑71 (MQ=255)aCGCTCAATCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCa > 1:1376679/1‑71 (MQ=255)aCGCTCAATCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCa > 1:1399126/1‑71 (MQ=255)aCGCTCAATCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCa > 1:1578025/1‑71 (MQ=255) aTCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTTATTGAgcg > 1:1145213/1‑71 (MQ=255) aTCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTTATTGAgcg > 1:3007303/1‑71 (MQ=255) aTCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTTATTGAgcg > 1:2145458/1‑71 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTGATTGAgcgc > 1:976520/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAgcgc > 1:1128566/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAgcgc > 1:3094919/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAgcgc > 1:3185051/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAgcgc > 1:2833006/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAgcgc > 1:59724/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAgcgc > 1:2228659/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAgcgc > 1:816414/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAgcgc > 1:2052510/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAgcgc > 1:863066/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAgcgc > 1:1841491/1‑70 (MQ=255) ctctTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCCTTCATTGAgcgc > 1:863730/1‑70 (MQ=255) ttCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTTATTGAGCGCaa < 1:359586/69‑1 (MQ=255) ttCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTTATTGAGCGCaa < 1:1843405/69‑1 (MQ=255) gcATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAGCGCAAAATGGCGCtt < 1:1456060/71‑1 (MQ=255) gTTTTCTTCGCCGGTCACACTGGTGATGAGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGaa < 1:2578793/71‑1 (MQ=255) gTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGaa < 1:2202364/71‑1 (MQ=255) gTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGaa < 1:3247915/71‑1 (MQ=255) gTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGaa < 1:3100758/71‑1 (MQ=255) ttttCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGaa < 1:1633663/70‑1 (MQ=255) cGGGTGATGAGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCTACAGaa < 1:7076/52‑1 (MQ=255) cGGGTGATGAGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGaa < 1:1147354/52‑1 (MQ=255) cGGGTGATGAGGGCATCGTCCATTGAGCGCAAAATGGCGCTTTTCAACAGaa < 1:1150657/52‑1 (MQ=255) tgatgaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGaa < 1:3260698/48‑1 (MQ=255) tgatgaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACg > 1:701057/1‑71 (MQ=255) tgatgaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACg > 1:1622568/1‑71 (MQ=255) tgatgaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACg > 1:1631359/1‑71 (MQ=255) tgatgaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACg > 1:2253175/1‑71 (MQ=255) tgatgaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACg > 1:2552475/1‑71 (MQ=255) tgatgaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACg > 1:2885623/1‑71 (MQ=255) gaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGgcg > 1:1954861/1‑51 (MQ=255) gaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACg > 1:1484138/1‑67 (MQ=255) gaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTa > 1:2118162/1‑70 (MQ=255) gaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTAt > 1:2907326/1‑71 (MQ=255) gaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTAt > 1:2287434/1‑71 (MQ=255) gaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTAt > 1:3000502/1‑71 (MQ=255) gaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTAt > 1:1153343/1‑71 (MQ=255) gaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTAt > 1:1837970/1‑71 (MQ=255) gaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTAt > 1:304926/1‑71 (MQ=255) gaGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTAt > 1:308254/1‑71 (MQ=255) cATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTATCCaa > 1:37804/1‑70 (MQ=255) cATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTATCCaa > 1:722513/1‑70 (MQ=255) ttCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCg < 1:3247923/54‑1 (MQ=255) ttCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCg < 1:3273808/54‑1 (MQ=255) ttCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCg < 1:2560268/54‑1 (MQ=255) ttCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCg < 1:399321/54‑1 (MQ=255) ttCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCg < 1:677646/54‑1 (MQ=255) ttCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCg < 1:1267972/54‑1 (MQ=255) | ACGCTCAATCTCTTCGAGGCGCATTTGCCCGTTTTCTTCGCCGGTCACACGGGTGATGAGGGCATCGTTCATTGAGCGCAAAATGGCGCTTTTCAACAGAAAATGGCGCGTTGCGAGATCGACGTTATCCAA > W3110S.gb/4086425‑4086556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |