| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,152,016 | C→T | 13.2% | E538K (GAG→AAG) | yhfK ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,152,016 | 0 | C | T | 13.2% | 181.4 / 16.8 | 68 | E538K (GAG→AAG) | yhfK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (21/38); new base T (4/5); total (25/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
ACAGAGTGTTATGTGCCTGATTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAC > W3110S.gb/4151950‑4152083 | aCAGAGTGTTATGTGCCTGATTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCt > 1:2527480/1‑70 (MQ=255)aCAGAGTGTTATGTGCCTGATTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCt > 1:318113/1‑70 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCTAATCGc < 1:3218729/69‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGc < 1:496810/69‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGc < 1:2339522/69‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGc < 1:3196441/69‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGc < 1:1211042/69‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGc < 1:1956431/69‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:1309073/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:2129403/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:1929530/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:3328783/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:1704309/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:2987415/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:899531/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:2192582/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:3098647/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:1492764/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:77908/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:1242398/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:638259/71‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGGTGCGGATCCTCGCTAAGAATCAAGCGAATCGc < 1:1526756/69‑1 (MQ=255) aTTTACCCGCATTCGCTGCCAGCCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:1528077/71‑1 (MQ=255) tttACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTc > 1:2769402/1‑71 (MQ=255) tttACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTc > 1:91089/1‑71 (MQ=255) tttACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTc > 1:749047/1‑71 (MQ=255) tttACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTc > 1:3253659/1‑71 (MQ=255) tttACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTc > 1:1136981/1‑71 (MQ=255) tttACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTc > 1:3300875/1‑71 (MQ=255) tttACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTc > 1:3304800/1‑71 (MQ=255) aCCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCt < 1:3318198/70‑1 (MQ=255) aCCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCt < 1:1193536/70‑1 (MQ=255) cccGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:885709/66‑1 (MQ=255) ttCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTTGCTAAGAATCAAGCGAATCGCTTcc < 1:1517923/62‑1 (MQ=255) ttCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTTGCTAAGAATCAAGCGAATCGCTTcc < 1:946771/62‑1 (MQ=255) ttCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTTGCTAAGAATCAAGCGAATCGCTTcc < 1:1168561/62‑1 (MQ=255) ttCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTTGCTAAGAATCAAGCGAATCGCTTcc < 1:3230556/62‑1 (MQ=255) ttCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTTGCTAAGAATCAAGCGAATCGCTTcc < 1:939772/62‑1 (MQ=255) ttCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCt < 1:35369/63‑1 (MQ=255) cGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTTGCTAAGAATCAAGCGAATCGCTTCCTg > 1:305079/1‑62 (MQ=255) cGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTTGCTAAGAATCAAGCGAATCGCTTCCTg > 1:3080792/1‑62 (MQ=255) cGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTTGCTAAGAATCAAGCGAATCGCTTCCTg > 1:1188908/1‑62 (MQ=255) cGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTTGCTAAGAATCAAGCGAATCGCTTCCTg > 1:1099576/1‑62 (MQ=255) gccaggccagTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:1666311/54‑1 (MQ=255) gCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGc < 1:645775/70‑1 (MQ=255) gCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGc < 1:2908067/70‑1 (MQ=255) gCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGc < 1:3289000/70‑1 (MQ=255) gCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGc < 1:1117095/70‑1 (MQ=255) gTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCtt < 1:1757016/40‑1 (MQ=255) ggTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGtttt > 1:354828/1‑70 (MQ=255) ggTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGtttt > 1:2888400/1‑70 (MQ=255) ggTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGtttt > 1:2880532/1‑70 (MQ=255) ggTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGtttt > 1:2198729/1‑70 (MQ=255) gTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGa < 1:2922294/41‑1 (MQ=255) gTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGa < 1:915567/41‑1 (MQ=255) gTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGa < 1:580942/41‑1 (MQ=255) gTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGa < 1:3311852/41‑1 (MQ=255) gTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGa < 1:2231430/41‑1 (MQ=255) ccTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAc > 1:2391991/1‑71 (MQ=255) ccTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAc > 1:1109220/1‑71 (MQ=255) ccTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAc > 1:1299682/1‑71 (MQ=255) ccTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAc > 1:254644/1‑71 (MQ=255) ccTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAc > 1:2701765/1‑71 (MQ=255) ccTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAc > 1:2895980/1‑71 (MQ=255) ccTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAc > 1:3327489/1‑71 (MQ=255) acTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAc > 1:1629843/2‑71 (MQ=255) cTCGCTAAGAATCAAGCTAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAc < 1:2217981/70‑1 (MQ=255) tCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAc < 1:122267/69‑1 (MQ=255) | ACAGAGTGTTATGTGCCTGATTTACCCGCATTCGCTGCCAGGCCAGTGGCGTAGGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAAC > W3110S.gb/4151950‑4152083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |