| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,154,666 | A→G | 37.5% | I23V (ATT→GTT) | yhfA → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,154,666 | 0 | A | G | 37.5% | 50.9 / 66.2 | 64 | I23V (ATT→GTT) | yhfA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (10/30); new base G (17/7); total (27/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.58e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGGCGGC > W3110S.gb/4154607‑4154730 | cgcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGAc > 1:2416432/1‑71 (MQ=255)cgcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGAc > 1:2419510/1‑71 (MQ=255)cgcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGAc > 1:2887649/1‑71 (MQ=255)cgcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGAc > 1:30792/1‑71 (MQ=255) gTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGtttt < 1:2207063/54‑1 (MQ=255) gTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGtttt < 1:556145/54‑1 (MQ=255) gTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGtttt < 1:1908587/54‑1 (MQ=255) gggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:2401586/1‑54 (MQ=255) gggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:2281591/1‑54 (MQ=255) gggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:2259300/1‑54 (MQ=255) gggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:601070/1‑54 (MQ=255) gggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:2625775/1‑54 (MQ=255) gggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:1817233/1‑54 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:1826527/1‑70 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:2244869/1‑70 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:3329345/1‑70 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:1642993/1‑70 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:553749/1‑70 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:714938/1‑70 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:74625/1‑70 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:1438527/1‑70 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:752259/1‑70 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:120807/1‑70 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:1156303/1‑70 (MQ=255) aGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAg < 1:2948485/71‑1 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCAcc < 1:3083837/71‑1 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCAcc < 1:1801350/71‑1 (MQ=255) ggCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGa < 1:2038212/71‑1 (MQ=255) ggCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGa < 1:2550077/71‑1 (MQ=255) ggCGAATCCGCCTCTGGTCATAAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTGCGATGGa < 1:1157253/71‑1 (MQ=255) gAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGaaa > 1:1921979/1‑70 (MQ=255) gAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAAt > 1:2089828/1‑71 (MQ=255) gAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAAt > 1:2016892/1‑71 (MQ=255) gAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAAt > 1:3124171/1‑71 (MQ=255) gAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAAt > 1:1999952/1‑71 (MQ=255) gAATCCGCCTATGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAAt > 1:2800138/1‑71 (MQ=255) gCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:807349/71‑1 (MQ=255) gCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:592274/71‑1 (MQ=255) gCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:1574116/71‑1 (MQ=255) gCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2830776/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATTGAAATGGTGtt < 1:712972/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:1598904/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:976678/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:1580612/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:580859/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:635652/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:1565848/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:1479952/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:125847/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:899173/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2336321/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:527272/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:178963/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:1900231/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2822413/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2803911/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2754449/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2729882/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2667578/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2142768/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2165540/71‑1 (MQ=255) gCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2309910/71‑1 (MQ=255) ccTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGGACCAAGTCCGATGGAAATGGTGtt < 1:50512/70‑1 (MQ=255) atcaGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATggcggc < 1:1810786/70‑1 (MQ=255) | CGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGGCGGC > W3110S.gb/4154607‑4154730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |