| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,185,304 | T→C | 6.6% | T95T (ACT→ACC) | gspA → | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,185,304 | 0 | T | C | 6.6% | 226.2 / 5.5 | 76 | T95T (ACT→ACC) | gspA | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base T (34/37); new base C (3/2); total (37/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
GTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTTGATG > W3110S.gb/4185243‑4185368 | gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACt > 1:1064567/1‑62 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCg > 1:2575097/1‑70 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:3028951/71‑1 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:1794089/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:1925885/71‑1 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:2190158/71‑1 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:2691581/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:2811358/71‑1 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:2947047/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:3003260/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:943621/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:3280343/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:3329947/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:3340749/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:6034/71‑1 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:655518/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:757666/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc > 1:1300117/1‑71 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:1181397/71‑1 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:1158538/71‑1 (MQ=255)gTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:1209058/71‑1 (MQ=255) tGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:1184003/70‑1 (MQ=255) tGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:2188894/70‑1 (MQ=255) tGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:3079716/70‑1 (MQ=255) tGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:399995/70‑1 (MQ=255) aGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACCGATAGTc < 1:1510205/64‑1 (MQ=255) aGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACCGATAGTc < 1:1040358/64‑1 (MQ=255) ggACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCttat > 1:2888046/1‑70 (MQ=255) atgagatgGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGaa < 1:1144589/71‑1 (MQ=255) tgagatgGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGaa < 1:2630893/70‑1 (MQ=255) agatgGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGc < 1:666908/47‑1 (MQ=255) aaTTACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGaa < 1:2969975/56‑1 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCttatta > 1:3146117/1‑45 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCttatta > 1:1146596/1‑45 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCttatta > 1:765875/1‑45 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCttatta > 1:1318116/1‑45 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCttatta > 1:1494479/1‑45 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCttatta > 1:1628153/1‑45 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGc > 1:3159849/1‑58 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGaa > 1:2435447/1‑67 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGaa > 1:2432284/1‑67 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGaa > 1:1068894/1‑67 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGaa > 1:34076/1‑67 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGaa > 1:3100257/1‑67 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGaa > 1:2713309/1‑67 (MQ=255) tACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGaa > 1:214729/1‑67 (MQ=255) tACTGCATGCGATTCATCGAACTGATAGTCGCTGccc > 1:694839/1‑37 (MQ=255) tACAGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGaa > 1:1992497/1‑67 (MQ=255) aCTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCAcc > 1:2552874/1‑71 (MQ=255) cATGCGCTTCATCGAACCGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCAccgccg > 1:2830555/1‑71 (MQ=255) cATGCGCTTCATCGAACCGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCAccgccg > 1:1987863/1‑71 (MQ=255) cATGCGCTTCATCGAACCGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCAccgccg > 1:1205741/1‑71 (MQ=255) cgcTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCTCAt < 1:109216/70‑1 (MQ=255) cgcTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCAt < 1:2158065/70‑1 (MQ=255) cgcTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCAt < 1:1511111/70‑1 (MQ=255) cgcTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCAt < 1:1240166/70‑1 (MQ=255) cgcTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCAt < 1:2587399/70‑1 (MQ=255) cgcTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCAt < 1:1105534/70‑1 (MQ=255) cgcTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATa > 1:732240/1‑71 (MQ=255) cgcTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATa > 1:2381697/1‑71 (MQ=255) cgcTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATa > 1:134415/1‑71 (MQ=255) aTCGTACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:1144196/70‑1 (MQ=255) aTCGAACTGATAGTCTCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:3314849/70‑1 (MQ=255) aTCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCTGCATACTtga < 1:854763/70‑1 (MQ=255) aTCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:1009426/70‑1 (MQ=255) aTCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:141925/70‑1 (MQ=255) aTCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:2600388/70‑1 (MQ=255) aTCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:1079097/70‑1 (MQ=255) aTCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:381944/70‑1 (MQ=255) aTCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:1617360/70‑1 (MQ=255) aTCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:2743870/70‑1 (MQ=255) aTCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:1147963/70‑1 (MQ=255) tCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtgatg < 1:369442/71‑1 (MQ=255) tCGAACTGATAGTCGCTGCCCTCTTATTATTACTGAAAATGCTCACCTGAATCACCGCCGCATACTtgatg < 1:2976153/71‑1 (MQ=255) cGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:547295/68‑1 (MQ=255) gAACTGATAGTCGCTGCCCTCTTATTATTATTGaaaa > 1:2641082/1‑37 (MQ=255) aaCTGATAGTAGCTGCCCTATTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTtga < 1:2625234/66‑1 (MQ=255) | GTGACAGCGCCTGGACGCGAGATGAGATGGCGGGGCAATTACTGCATGCGCTTCATCGAACTGATAGTCGCTGCCCTCTTATTATTATTGAAAATGCTCACCTGAATCACCGCCGCATACTTGATG > W3110S.gb/4185243‑4185368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |