| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,217,545 | A→G | 12.6% | N57N (AAT→AAC) | yjaB ← | predicted acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,217,545 | 0 | A | G | 12.6% | 211.1 / 19.5 | 79 | N57N (AAT→AAC) | yjaB | predicted acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (47/22); new base G (8/2); total (55/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CATATGCTGCCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTCCGA > W3110S.gb/4217497‑4217611 | cATATGCTGCCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCGTTAACCGCGACCCACAACGgc < 1:1528757/70‑1 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCGTTAACCGCGa > 1:807998/1‑55 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCGTTAACCGCGACCCACAACGGCGCTTc > 1:93796/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCGTTAACCGCGACCCACAACGGCGCTTc > 1:2888568/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCGTTAACCGCGACCCACAACGGCGCTTc > 1:447751/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCGTTAACCGCGACCCACAACGGCGCTTc > 1:2273817/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCGTTAACCGCGACCCACAACGGCGCTTc > 1:2344/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCGTTAACCGCGACCCACAACGGCGCTTc > 1:1437890/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCGTTAACCGCGACCCACAACGGCGCTTc > 1:327937/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACgg > 1:2158906/1‑65 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTc > 1:1720882/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTc > 1:1561834/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTc > 1:181357/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTc > 1:1494267/1‑71 (MQ=255) tgctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTc > 1:844411/1‑71 (MQ=255) ctgcCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCg > 1:1381287/1‑71 (MQ=255) tAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaag > 1:1310387/1‑70 (MQ=255) tAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaag > 1:2637259/1‑70 (MQ=255) tAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaag > 1:684702/1‑70 (MQ=255) aGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaagg > 1:455489/1‑70 (MQ=255) aGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaagg > 1:1988922/1‑70 (MQ=255) aGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaagg > 1:172536/1‑70 (MQ=255) aGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaagg > 1:1961808/1‑70 (MQ=255) aGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaagg > 1:695204/1‑70 (MQ=255) aGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaagg > 1:3288434/1‑70 (MQ=255) aGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaagg > 1:3236387/1‑70 (MQ=255) aGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCaggaagg > 1:3171323/1‑70 (MQ=255) aGCAACATAAATCCAACCGGCTGATCCCGCTCAATaa > 1:1706183/1‑37 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:2234336/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:2614875/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:273111/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:99493/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:1912220/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:1221764/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:1222776/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:1279727/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:879929/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:1539459/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:83372/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGa > 1:1875821/1‑41 (MQ=255) cAACATAAATCCAACCGGCTGATCCCGCTCATTAAACGCGa > 1:376173/1‑41 (MQ=255) aCATAAATCCAACCGGCTGCTCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacg > 1:3210507/1‑70 (MQ=255) aCATAAATCCAACCGGCTGATCCCGCTCGTTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacga < 1:1442549/71‑1 (MQ=255) aCATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGCCCCACAACGGCGCTTCCGGCAGGAAGgaacg > 1:2013956/1‑70 (MQ=255) aCATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacg > 1:619786/1‑70 (MQ=255) aCATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacg > 1:2785460/1‑70 (MQ=255) aTAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac < 1:1542998/71‑1 (MQ=255) aTAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac < 1:3212089/71‑1 (MQ=255) aTAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac < 1:1265739/71‑1 (MQ=255) aTAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac < 1:3207871/71‑1 (MQ=255) tAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCAGAACGGCGCTTCCGGCAGGAAGgaacgaac > 1:2979496/1‑70 (MQ=255) tAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac > 1:499543/1‑70 (MQ=255) tAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac > 1:1093974/1‑70 (MQ=255) tAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac > 1:3198437/1‑70 (MQ=255) tAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac > 1:3221117/1‑70 (MQ=255) tAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac > 1:797948/1‑70 (MQ=255) tAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac < 1:3049754/70‑1 (MQ=255) tAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac > 1:2556228/1‑70 (MQ=255) tAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGgaacgaac < 1:2030461/70‑1 (MQ=255) aaTCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAg < 1:192768/71‑1 (MQ=255) aaTCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAg < 1:1305437/71‑1 (MQ=255) aaTCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAg < 1:1613050/71‑1 (MQ=255) aaTCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAg < 1:738579/71‑1 (MQ=255) aaTCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAg < 1:1616868/71‑1 (MQ=255) aaTCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAg < 1:173119/71‑1 (MQ=255) aaTCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAg < 1:1831/71‑1 (MQ=255) aaTCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAg < 1:3187232/71‑1 (MQ=255) aaTCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAg < 1:2244893/71‑1 (MQ=255) aTCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAgg < 1:2016843/71‑1 (MQ=255) ccGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTcc < 1:1994939/71‑1 (MQ=255) ccGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTcc < 1:2555017/71‑1 (MQ=255) ccGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTcc < 1:1170849/71‑1 (MQ=255) ccGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTcc < 1:1003682/71‑1 (MQ=255) gCTCATTAACCTCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTcc < 1:1541013/69‑1 (MQ=255) cTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTCCGa > 1:2359895/1‑70 (MQ=255) cTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTCCGa > 1:2498773/1‑70 (MQ=255) cTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTCCGa > 1:1593740/1‑70 (MQ=255) cTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTCCGa > 1:1396267/1‑70 (MQ=255) cATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAg < 1:2893506/49‑1 (MQ=255) | CATATGCTGCCCACTTAGCAACATAAATCCAACCGGCTGATCCCGCTCATTAACCGCGACCCACAACGGCGCTTCCGGCAGGAAGGAACGAACTAGGTCCTCCAGCTCGGTCCGA > W3110S.gb/4217497‑4217611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |