Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 85,966 T→C 12.8% F113L (TTT→CTT)  ilvI → acetolactate synthase III, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb85,9660TC12.8% 184.2 / 15.9 70F113L (TTT→CTT) ilvIacetolactate synthase III, large subunit
Reads supporting (aligned to +/- strand):  ref base T (17/44);  new base C (7/2);  total (24/46)
Fisher's exact test for biased strand distribution p-value = 6.05e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCA  >  W3110S.gb/85903‑86030
                                                               |                                                                
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAgg                                                            <  1:913649/70‑1 (MQ=255)
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAgg                                                            <  1:3255246/70‑1 (MQ=255)
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAgg                                                            <  1:1737774/70‑1 (MQ=255)
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAgg                                                            <  1:625531/70‑1 (MQ=255)
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAgg                                                            <  1:72889/70‑1 (MQ=255)
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAgg                                                            <  1:2814638/70‑1 (MQ=255)
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAgg                                                            <  1:821923/70‑1 (MQ=255)
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAgg                                                            <  1:89170/70‑1 (MQ=255)
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACCATGCCTTTCAgg                                                            <  1:3321662/70‑1 (MQ=255)
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGAACTCGTTGATAGGTTACGATGCCTTTCAgg                                                            <  1:1648581/70‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:2550557/60‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:2325377/60‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:3186655/60‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:2041541/60‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:320887/60‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:2013185/60‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:3318302/60‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:1971479/60‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:3329491/60‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:460628/60‑1 (MQ=255)
       ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:1544256/60‑1 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTc                                                               <  1:2554511/58‑1 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAg                                                             >  1:3219145/1‑60 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAg                                                             >  1:368989/1‑60 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAg                                                             >  1:3039202/1‑60 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAg                                                             >  1:299857/1‑60 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAg                                                             >  1:587714/1‑60 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAg                                                             >  1:2675175/1‑60 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAg                                                             >  1:2522751/1‑60 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAg                                                             >  1:1177423/1‑60 (MQ=255)
         ccattAGTTGTCCTTTCAGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAg                                                             >  1:1088662/1‑60 (MQ=255)
                  gTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAtggtg                                              >  1:2640138/1‑66 (MQ=255)
                  gTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGAt                                         >  1:2838438/1‑71 (MQ=255)
                  gTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGAt                                         >  1:1647603/1‑71 (MQ=255)
                  gTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGAt                                         >  1:2140414/1‑71 (MQ=255)
                  gTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGAt                                         >  1:1671154/1‑71 (MQ=255)
                  gTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGAt                                         >  1:1987779/1‑71 (MQ=255)
                  gTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTAAGATGCCCTTCAGGAGTGCGACATGGTGGGGAt                                         >  1:470360/1‑71 (MQ=255)
                            ggCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:1136547/71‑1 (MQ=255)
                            ggCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:849404/71‑1 (MQ=255)
                            ggCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:1292653/71‑1 (MQ=255)
                            ggCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:1594714/71‑1 (MQ=255)
                            ggCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:72242/71‑1 (MQ=255)
                            ggCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:1599324/71‑1 (MQ=255)
                            ggCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:2118338/71‑1 (MQ=255)
                            ggCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:2741638/71‑1 (MQ=255)
                            ggCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:2319639/71‑1 (MQ=255)
                            ggCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:3055743/71‑1 (MQ=255)
                                        ccTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAacac                   <  1:2568516/71‑1 (MQ=255)
                                        ccTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAacac                   <  1:3224703/71‑1 (MQ=255)
                                        ccTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAacac                   <  1:3317773/71‑1 (MQ=255)
                                        ccTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAacac                   <  1:1827305/71‑1 (MQ=255)
                                        ccTCGTTGATAGGTTACGATACCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAacac                   <  1:1778448/71‑1 (MQ=255)
                                               gATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGa                                  >  1:3336083/1‑49 (MQ=255)
                                               gATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTc            >  1:3119362/1‑71 (MQ=255)
                                               gATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTc            >  1:2496344/1‑71 (MQ=255)
                                               gATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTc            >  1:2443073/1‑71 (MQ=255)
                                               gATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTc            >  1:430546/1‑71 (MQ=255)
                                               gATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTc            >  1:1777547/1‑71 (MQ=255)
                                               gATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTc            >  1:1965531/1‑71 (MQ=255)
                                               gATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTc            >  1:3066877/1‑71 (MQ=255)
                                                    gTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtggt                          <  1:2946044/52‑1 (MQ=255)
                                                    gTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtggt                          <  1:1995428/52‑1 (MQ=255)
                                                    gTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtggt                          <  1:802927/52‑1 (MQ=255)
                                                    gTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtggt                          <  1:2636962/52‑1 (MQ=255)
                                                    gTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtggt                          <  1:1048619/52‑1 (MQ=255)
                                                         gATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:396850/42‑1 (MQ=255)
                                                         gATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCa  <  1:2581156/71‑1 (MQ=255)
                                                         gATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCa  <  1:2023691/71‑1 (MQ=255)
                                                            gCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCg                               <  1:94086/39‑1 (MQ=255)
                                                               |                                                                
GATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCA  >  W3110S.gb/85903‑86030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: