| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,254,714 | A→T | 32.9% | E56D (GAA→GAT) | yjbI → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,254,714 | 0 | A | T | 32.9% | 86.6 / 67.2 | 70 | E56D (GAA→GAT) | yjbI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (20/27); new base T (10/13); total (30/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01 | |||||||||||
AAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGAC > W3110S.gb/4254644‑4254772 | aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:1657748/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:3305646/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:3211941/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:367695/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:2929089/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:2399597/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:1751943/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:373410/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:1460253/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:495814/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:2549263/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:889795/71‑1 (MQ=255)aaGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:1125570/71‑1 (MQ=255) ggTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:2099084/69‑1 (MQ=255) gTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:3092776/68‑1 (MQ=255) gTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:902748/68‑1 (MQ=255) gACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTgg > 1:2411321/1‑71 (MQ=255) gACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTgg > 1:720508/1‑71 (MQ=255) gACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTgg > 1:2518032/1‑71 (MQ=255) gACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTgg > 1:1975031/1‑71 (MQ=255) gACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTgg > 1:2534260/1‑71 (MQ=255) gACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTgg > 1:2889456/1‑71 (MQ=255) gACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTgg > 1:2233818/1‑71 (MQ=255) gACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTgg > 1:3094591/1‑71 (MQ=255) gACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTgg > 1:3214249/1‑71 (MQ=255) atatCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTg > 1:587673/1‑71 (MQ=255) atatCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTg > 1:1569142/1‑71 (MQ=255) atCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:1991093/58‑1 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:942834/57‑1 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:2610663/57‑1 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGaa < 1:2007131/57‑1 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCaa > 1:1198195/1‑71 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCaa > 1:1878593/1‑71 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCaa > 1:2133871/1‑71 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCaa > 1:841152/1‑71 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCaa > 1:2873563/1‑71 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCaa > 1:365655/1‑71 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCaa > 1:2433507/1‑71 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCaa > 1:2988373/1‑71 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCaa > 1:1821105/1‑71 (MQ=255) tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCaa > 1:1829635/1‑71 (MQ=255) gAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAAt < 1:833413/70‑1 (MQ=255) ggTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTta > 1:2374959/1‑71 (MQ=255) ggTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTta > 1:2369428/1‑71 (MQ=255) ggTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTta > 1:3208742/1‑71 (MQ=255) gtgtCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTtac < 1:18540/71‑1 (MQ=255) gtgtCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTtac < 1:1858450/71‑1 (MQ=255) gtgtCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTtac < 1:2188206/71‑1 (MQ=255) gtgtCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTtac < 1:1935894/71‑1 (MQ=255) gtgtCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTtac < 1:2551314/71‑1 (MQ=255) tgtCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTtac > 1:2026660/1‑70 (MQ=255) tgtCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTtac < 1:1833467/70‑1 (MQ=255) tgtCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTtac > 1:13809/1‑70 (MQ=255) tgtCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTtac > 1:2804058/1‑70 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:284261/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:2406928/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:2817070/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:1100112/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:1455453/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:1370845/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:2194188/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:2252787/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:2078048/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:1039346/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:2533357/70‑1 (MQ=255) ttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:1172028/70‑1 (MQ=255) aaaTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGAt > 1:2116550/1‑70 (MQ=255) aaTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATaa < 1:1866193/71‑1 (MQ=255) aaTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATaa < 1:1236381/71‑1 (MQ=255) aaTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATaa < 1:1240139/71‑1 (MQ=255) aaTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATaa < 1:2538885/71‑1 (MQ=255) aaTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATaa < 1:3085320/71‑1 (MQ=255) aaTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATaa < 1:2707481/71‑1 (MQ=255) aaTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATaa < 1:2808640/71‑1 (MQ=255) aaTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATaa < 1:646869/71‑1 (MQ=255) aaTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGAATTGCAATTTTACTACTGCAATAGTTGATaa < 1:1634861/71‑1 (MQ=255) tCTACAACATGTAACATTCGAATGTAAAGATATTGGGGATTGCAATTTTACTACTGc < 1:3304796/57‑1 (MQ=255) aaCATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATaa < 1:2864177/57‑1 (MQ=255) aCATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATg < 1:1289296/71‑1 (MQ=255) aCATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATg < 1:1088436/71‑1 (MQ=255) aCATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATg < 1:3270107/71‑1 (MQ=255) aCATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATg < 1:2177659/71‑1 (MQ=255) aCATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATg < 1:298486/71‑1 (MQ=255) aCATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATg < 1:2740556/71‑1 (MQ=255) ttCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAAt < 1:1858553/54‑1 (MQ=255) ttCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAAt < 1:2103648/54‑1 (MQ=255) ttCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAAt < 1:2303125/54‑1 (MQ=255) ttCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAAt < 1:2311179/54‑1 (MQ=255) cGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGa > 1:2775943/1‑70 (MQ=255) cGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGAc > 1:2764655/1‑71 (MQ=255) | AAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGAC > W3110S.gb/4254644‑4254772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |