| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,338,229 | C→T | 6.7% | L134L (CTG→CTA) | basR ← | DNA‑binding response regulator in two‑component regulatory system with BasS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,338,229 | 0 | C | T | 6.7% | 234.4 / 5.1 | 74 | L134L (CTG→CTA) | basR | DNA‑binding response regulator in two‑component regulatory system with BasS |
| Reads supporting (aligned to +/- strand): ref base C (36/33); new base T (3/2); total (39/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
AGAGCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGA > W3110S.gb/4338161‑4338296 | agagCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAg < 1:3332249/71‑1 (MQ=255)agagCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAg < 1:2979973/71‑1 (MQ=255)agagCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAg < 1:1470223/71‑1 (MQ=255)agagCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAg < 1:512225/71‑1 (MQ=255)agagCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAg < 1:3000301/71‑1 (MQ=255)agagCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAg < 1:656276/71‑1 (MQ=255) cATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTTAGCGt > 1:2298204/1‑70 (MQ=255) cATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTTAGCGt > 1:1493647/1‑70 (MQ=255) cATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTTAGCGt > 1:624926/1‑70 (MQ=255) tatTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAg < 1:2729282/71‑1 (MQ=255) tatTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAg < 1:3223513/71‑1 (MQ=255) atTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGt > 1:2121622/1‑67 (MQ=255) atTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGt > 1:12687/1‑67 (MQ=255) atTCTTTGGGCGTCAGAAT‑AACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGAt > 1:3045298/1‑71 (MQ=255) tGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGcc > 1:1504560/1‑70 (MQ=255) tGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGcc > 1:2783735/1‑70 (MQ=255) gCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTTAGCGTCAGATTGCcaaca < 1:2157336/71‑1 (MQ=255) cGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTTAGCGTCAGATTGCcaaca < 1:2305774/70‑1 (MQ=255) aGAATCTACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAg > 1:1856330/1‑71 (MQ=255) aGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAg > 1:3243969/1‑71 (MQ=255) aGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAg > 1:3070787/1‑71 (MQ=255) aGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAg > 1:486379/1‑71 (MQ=255) tCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCa < 1:2810531/71‑1 (MQ=255) tCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCa < 1:1228902/71‑1 (MQ=255) tCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCa < 1:2880874/71‑1 (MQ=255) tCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCa < 1:897971/71‑1 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCcaa > 1:2853739/1‑51 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCcaa > 1:2790465/1‑51 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCca > 1:3083225/1‑50 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGccc > 1:2858448/1‑71 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGccc > 1:3217575/1‑71 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGccc > 1:384848/1‑71 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGccc > 1:2625450/1‑71 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGccc > 1:499960/1‑71 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGccc > 1:217200/1‑71 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGccc > 1:541327/1‑71 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGccc > 1:1965757/1‑71 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGccc > 1:1725373/1‑71 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGccc > 1:926700/1‑71 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGcc > 1:2736842/1‑70 (MQ=255) cACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGcc > 1:2637740/1‑70 (MQ=255) cACCGCCCATCCATACATGACGGCGACCCATGTTCAGCGTCAGATTGCcaa > 1:617341/1‑51 (MQ=255) gCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGat < 1:2665527/71‑1 (MQ=255) catACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGatta > 1:2695821/1‑66 (MQ=255) catACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATg > 1:3322909/1‑71 (MQ=255) catACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATg > 1:3259959/1‑71 (MQ=255) catACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATg > 1:958799/1‑71 (MQ=255) catACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATg > 1:1976707/1‑71 (MQ=255) catACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATg > 1:2084486/1‑71 (MQ=255) catACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATg > 1:2749635/1‑71 (MQ=255) catACCTGACGGCGACCCATGTTCAGCGTCAGATTG‑CAACAATCAGCTCACTTTCGCCCTGATTATTATg > 1:492191/1‑70 (MQ=255) catACCTGACGGCGACCCAAGTTCAGCGTCAGATTGCCAACAATCAg > 1:2159/1‑47 (MQ=255) aCGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTa < 1:1537785/71‑1 (MQ=255) aCGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTa < 1:1571622/71‑1 (MQ=255) aCGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTa < 1:3177172/71‑1 (MQ=255) aCGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTa < 1:516902/71‑1 (MQ=255) gCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCa < 1:1107114/71‑1 (MQ=255) gCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCa < 1:860955/71‑1 (MQ=255) gCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCa < 1:658950/71‑1 (MQ=255) gCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCa < 1:36840/71‑1 (MQ=255) tGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGAttatta > 1:3069116/1‑50 (MQ=255) tGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGc < 1:2376235/62‑1 (MQ=255) tGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGc < 1:771912/62‑1 (MQ=255) tGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGc < 1:2240658/62‑1 (MQ=255) tGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGc < 1:297482/62‑1 (MQ=255) tGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGcgcg > 1:3094921/1‑70 (MQ=255) ttCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGa < 1:484426/70‑1 (MQ=255) ttCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGa < 1:2791970/70‑1 (MQ=255) ttCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGa < 1:359383/70‑1 (MQ=255) ttCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGa < 1:3347048/70‑1 (MQ=255) ttCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGa < 1:2907682/70‑1 (MQ=255) ttCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGa < 1:883737/70‑1 (MQ=255) ttCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGa < 1:3310299/70‑1 (MQ=255) ttCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGa < 1:1286122/70‑1 (MQ=255) | AGAGCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGA > W3110S.gb/4338161‑4338296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |