| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,430,000 | G→A | 14.1% | P67P (CCG→CCA) | rpsF → | 30S ribosomal subunit protein S6 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,430,000 | 0 | G | A | 14.1% | 203.0 / 20.8 | 78 | P67P (CCG→CCA) | rpsF | 30S ribosomal subunit protein S6 |
| Reads supporting (aligned to +/- strand): ref base G (57/10); new base A (6/5); total (63/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.13e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGT > W3110S.gb/4429936‑4430065 | aGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAgg > 1:1181362/1‑69 (MQ=255)aGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAg > 1:1954832/1‑68 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGTTCCGCAGGAAGTGATCGATGAGCTg > 1:883879/1‑70 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:2522507/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:833268/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:75863/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:338792/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:307103/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:2908499/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:281401/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:2594818/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:2238559/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:1613852/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGa > 1:158590/1‑63 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTg > 1:638812/1‑70 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTg > 1:3209836/1‑70 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTg > 1:3324938/1‑70 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTg > 1:2747232/1‑70 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTg > 1:3614/1‑70 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTg > 1:2565239/1‑70 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTg > 1:2157222/1‑70 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTg > 1:2070897/1‑70 (MQ=255) caacaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTg > 1:183065/1‑70 (MQ=255) caacaaACAGCACAAAGCACACTACGTTCTGATGAATGTTGAAGATCCGCAGCAAGTGATAGATGAGCTg > 1:1676856/1‑70 (MQ=255) acaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATc < 1:1284846/59‑1 (MQ=255) acaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATc < 1:285384/59‑1 (MQ=255) acaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATc < 1:1698765/59‑1 (MQ=255) acaaACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATc < 1:1630117/59‑1 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATg > 1:1800797/1‑60 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:182065/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:3084679/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:1288295/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:2870873/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:2752209/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:2599262/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:366759/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:388688/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:2528136/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:1061403/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:2406040/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:1768935/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:1428306/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:762700/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:2512040/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:2000696/1‑70 (MQ=255) aaCTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAAc > 1:2001032/1‑70 (MQ=255) aaCAGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGc > 1:3191906/1‑63 (MQ=255) aaGCACACTACGTTCTGATGACTGTTGAAGCTCCGCAGGAAGTGATc < 1:1567453/47‑1 (MQ=255) aGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCt > 1:755980/1‑71 (MQ=255) aGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCt > 1:1143513/1‑71 (MQ=255) tACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGa > 1:2771711/1‑71 (MQ=255) tACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGa > 1:1619041/1‑71 (MQ=255) tACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGa > 1:2838894/1‑71 (MQ=255) tACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGa > 1:1287151/1‑71 (MQ=255) tACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGa > 1:648857/1‑71 (MQ=255) tACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGa > 1:592525/1‑71 (MQ=255) ttCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCTATGAGCTGGa < 1:1515446/46‑1 (MQ=255) ttCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGa < 1:1597590/46‑1 (MQ=255) ttCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGa < 1:2930125/46‑1 (MQ=255) gatgaATGTTGAAGCTCCACAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGtt > 1:2604546/1‑70 (MQ=255) gatgaATGTTGAAGCTCCACAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTa > 1:1974582/1‑71 (MQ=255) gatgaATGTTGAAGCTCCACAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTa > 1:2575065/1‑71 (MQ=255) gatgaATGTTGAAGCTCCACAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTa > 1:22654/1‑71 (MQ=255) gatgaATGTTGAAGCTCCACAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTa > 1:563158/1‑71 (MQ=255) gatgaATGTTGAAGCTCCACAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTa > 1:2413696/1‑71 (MQ=255) ttGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCgcagc < 1:434037/71‑1 (MQ=255) gAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCg > 1:3266189/1‑65 (MQ=255) gAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCg > 1:841643/1‑65 (MQ=255) gAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCg > 1:1222507/1‑65 (MQ=255) gAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCg > 1:438869/1‑65 (MQ=255) gAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCg > 1:1378760/1‑65 (MQ=255) gAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCg > 1:1315343/1‑65 (MQ=255) gCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGt < 1:2318933/71‑1 (MQ=255) gCTCCACAGGAAGTGATCGATGATCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGt < 1:1327909/71‑1 (MQ=255) gCTCCACAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGt < 1:624568/71‑1 (MQ=255) gCTCCACAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGt < 1:2183989/71‑1 (MQ=255) gCTCCACAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGt < 1:1729260/71‑1 (MQ=255) gCTCCACAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGt < 1:1625289/71‑1 (MQ=255) | AGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGT > W3110S.gb/4429936‑4430065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |