Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	155,463	A→T	100%	*247K (TAA→AAA)	ecpD ←	predicted periplasmic pilin chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	155,463	0	A	T	100.0%	72.5 / NA	23	*247K (TAA→AAA)	ecpD	predicted periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/0);  total (23/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGC  >  W3110S.gb/155396‑155487                                                                    |                         gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg                       >  1:1061941/1‑71 (MQ=255) gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg                       >  1:571140/1‑71 (MQ=255) gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg                       >  1:2679183/1‑71 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg                       >  1:2724087/1‑66 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:754164/1‑67 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:1024480/1‑67 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:541618/1‑67 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:3235634/1‑67 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:2886082/1‑67 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:2408712/1‑67 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:2374318/1‑67 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:2369054/1‑67 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:2152703/1‑67 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:1811141/1‑67 (MQ=255)      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc                      >  1:1700573/1‑67 (MQ=255)                      cTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc  >  1:1996877/1‑71 (MQ=255)                                   aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTa             >  1:2416140/1‑47 (MQ=255)                                   aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc            >  1:2309664/1‑48 (MQ=255)                                   aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc            >  1:2002865/1‑48 (MQ=255)                                   aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc            >  1:1821358/1‑48 (MQ=255)                                   aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc            >  1:524502/1‑48 (MQ=255)                                   aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc            >  1:1719183/1‑48 (MQ=255)                                   aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc            >  1:1203491/1‑48 (MQ=255)                                                                    |                         GATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGC  >  W3110S.gb/155396‑155487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑