Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	230,040	C→A	28.5%	R281L (CGC→CTC)	yafC ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	230,040	0	C	A	28.5%	53.7 / 13.9	28	R281L (CGC→CTC)	yafC	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (14/6);  new base A (4/4);  total (18/10)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

AGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTC  >  W3110S.gb/229982‑230089                                                           |                                                  aGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCg                                       >  1:198016/1‑71 (MQ=255)               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGt                           >  1:2424133/1‑69 (MQ=255)               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTaa                         >  1:889612/1‑71 (MQ=255)               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTaa                         >  1:3041321/1‑71 (MQ=255)               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTaa                         >  1:2593620/1‑71 (MQ=255)               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTaa                         >  1:1480273/1‑71 (MQ=255)               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTaa                         >  1:2414052/1‑71 (MQ=255)               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTaa                         >  1:1676815/1‑71 (MQ=255)               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTaa                         >  1:1902373/1‑71 (MQ=255)               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTaa                         >  1:1790737/1‑71 (MQ=255)               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTa                          >  1:943582/1‑70 (MQ=255)                  tttttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTaa                         >  1:3139006/1‑68 (MQ=255)                  tttttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTaa                         >  1:2810734/1‑68 (MQ=255)                       aCATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACgg                  <  1:104491/70‑1 (MQ=255)                            cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg             >  1:1377758/1‑70 (MQ=255)                            cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg             >  1:1252751/1‑70 (MQ=255)                            cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg             >  1:934709/1‑70 (MQ=255)                            cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTAACCGCACGGTCGCTGTAAt                        >  1:2475762/1‑59 (MQ=255)                                cTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATgg        <  1:1503330/71‑1 (MQ=255)                                cTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATgg        <  1:2892814/71‑1 (MQ=255)                                cTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATgg        <  1:3263400/71‑1 (MQ=255)                                cTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATgg        <  1:734657/71‑1 (MQ=255)                                cTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATgg        <  1:1736187/71‑1 (MQ=255)                                 tAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATgg        <  1:1625301/70‑1 (MQ=255)                                      aaaTCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTc  <  1:2550166/71‑1 (MQ=255)                                      aaaTCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTc  <  1:234360/71‑1 (MQ=255)                                      aaaTCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTc  <  1:1124052/71‑1 (MQ=255)                                              aaaaGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGa                     >  1:1084508/1‑44 (MQ=255)                                                           |                                                  AGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTC  >  W3110S.gb/229982‑230089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑