| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 234,234 | C→T | 56.5% | L183L (TTG→TTA) | gloB ← | predicted hydroxyacylglutathione hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 234,234 | 0 | C | T | 56.5% | 21.2 / 76.6 | 62 | L183L (TTG→TTA) | gloB | predicted hydroxyacylglutathione hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (15/12); new base T (9/26); total (24/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCACAA > W3110S.gb/234171‑234297 | gggTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGt < 1:1226639/71‑1 (MQ=255)gggTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGt < 1:927178/71‑1 (MQ=255)gggTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGt < 1:2473412/71‑1 (MQ=255) gtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCgg > 1:950228/1‑68 (MQ=255) gtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCgg > 1:74521/1‑68 (MQ=255) gtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCgg > 1:2365931/1‑68 (MQ=255) gtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCgg > 1:1373728/1‑68 (MQ=255) gtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCgg > 1:2607684/1‑68 (MQ=255) gtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCgg > 1:851530/1‑68 (MQ=255) gtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCgg > 1:1509281/1‑68 (MQ=255) gtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCg > 1:1756939/1‑67 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:396577/70‑1 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:2686320/70‑1 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:2249025/70‑1 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:1953803/70‑1 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:2729805/70‑1 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:3180947/70‑1 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:1453479/70‑1 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:1181986/70‑1 (MQ=255) tGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCa < 1:2809092/70‑1 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:2633045/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:911035/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:1209539/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:1500598/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:2426094/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:575049/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:833757/1‑71 (MQ=255) tCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATAtt > 1:2780242/1‑71 (MQ=255) tCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATAtt > 1:656373/1‑71 (MQ=255) tCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATAtt > 1:1946463/1‑71 (MQ=255) ccTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACtt > 1:2459564/1‑64 (MQ=255) ccTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATAttt > 1:2261514/1‑71 (MQ=255) ccTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATAttt > 1:1622991/1‑71 (MQ=255) ccTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATAttt > 1:1740269/1‑71 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAATGt < 1:1729496/70‑1 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGt < 1:1154414/70‑1 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGt < 1:3289544/70‑1 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGt < 1:525419/70‑1 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGt < 1:527806/70‑1 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGt < 1:847256/70‑1 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGt < 1:694707/70‑1 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGt < 1:231260/70‑1 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGt < 1:1841572/70‑1 (MQ=255) aCGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:1050749/70‑1 (MQ=255) cGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:2553358/69‑1 (MQ=255) cGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:1837734/69‑1 (MQ=255) cGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:663034/69‑1 (MQ=255) aatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCAt > 1:1558832/1‑69 (MQ=255) aatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCAt > 1:1617018/1‑69 (MQ=255) tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca < 1:2164047/71‑1 (MQ=255) tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca < 1:1381375/71‑1 (MQ=255) tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca < 1:901560/71‑1 (MQ=255) tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca < 1:1835132/71‑1 (MQ=255) tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca < 1:1281653/71‑1 (MQ=255) tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca < 1:51548/71‑1 (MQ=255) tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca < 1:487239/71‑1 (MQ=255) tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca < 1:259530/71‑1 (MQ=255) tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca < 1:3158033/71‑1 (MQ=255) tATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa < 1:96281/70‑1 (MQ=255) tATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa < 1:3063048/70‑1 (MQ=255) aTGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa < 1:2402248/69‑1 (MQ=255) tGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa < 1:2459309/68‑1 (MQ=255) | GGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCACAA > W3110S.gb/234171‑234297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |