| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 344,381 | G→A | 33.3% | intergenic (+166/‑247) | yahL → / → yahM | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 344,381 | 0 | G | A | 33.3% | 36.7 / 25.5 | 34 | intergenic (+166/‑247) | yahL/yahM | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (12/10); new base A (6/5); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCA > W3110S.gb/344314‑344451 | ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:706240/1‑71 (MQ=255) tctTTTTTCGCCGATTTTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:2142749/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:818267/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:3298562/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:2970511/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:434916/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:2498473/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:2413455/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:2049195/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:1618211/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:1612585/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:1441410/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:660690/1‑71 (MQ=255) atTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCAt > 1:2219863/1‑60 (MQ=255) atTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGtata > 1:411427/1‑70 (MQ=255) atTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGtata > 1:3221213/1‑70 (MQ=255) atTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGtata > 1:394547/1‑70 (MQ=255) atTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGtata > 1:1543625/1‑70 (MQ=255) ttgttgTGATCGGCGCCACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATaaaa < 1:3075490/70‑1 (MQ=255) ttgttgTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATaaaa < 1:2545898/70‑1 (MQ=255) ttgttgTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATaaaa < 1:2734515/70‑1 (MQ=255) ttgttgTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATaaaa < 1:2462708/70‑1 (MQ=255) cGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCa < 1:2887864/71‑1 (MQ=255) cTTCGCTTAAAAAAGCACCAGTAGTGCTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:3132975/71‑1 (MQ=255) cTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:48531/71‑1 (MQ=255) cTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:507315/71‑1 (MQ=255) cTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:2862093/71‑1 (MQ=255) gcAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:2699345/53‑1 (MQ=255) gtagtGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGa < 1:510557/71‑1 (MQ=255) gtagtGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGa < 1:707296/71‑1 (MQ=255) gtagtGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGa < 1:851985/71‑1 (MQ=255) gtagtGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGa < 1:1871051/71‑1 (MQ=255) gtagtGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGa < 1:1843606/71‑1 (MQ=255) gtagtGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGa < 1:1548117/71‑1 (MQ=255) gTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCa < 1:2543238/71‑1 (MQ=255) gTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCa < 1:1392356/71‑1 (MQ=255) gTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCa < 1:1174099/71‑1 (MQ=255) | CCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCA > W3110S.gb/344314‑344451 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |