| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 47,828 | A→G | 36.8% | V20V (GTA→GTG) | kefC → | potassium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 47,828 | 0 | A | G | 36.8% | 43.6 / 57.9 | 57 | V20V (GTA→GTG) | kefC | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base A (19/17); new base G (14/7); total (33/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01 | |||||||||||
GGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCC > W3110S.gb/47761‑47895 | ggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc > 1:2945001/1‑70 (MQ=255)ggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc > 1:1094658/1‑70 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTg < 1:965919/69‑1 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTg < 1:2531447/69‑1 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTg < 1:1790265/69‑1 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTg < 1:172518/69‑1 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTg < 1:3256834/69‑1 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATT‑TACCCATTGCgg < 1:3040908/71‑1 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTGCGGCAGCGCTGATTGTGCCCATTg < 1:503364/69‑1 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATATATCTCGGTTCGGCAGCGCTGATTGTGCCCATTg < 1:3272307/69‑1 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg < 1:3291115/70‑1 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg < 1:487892/70‑1 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg < 1:2054535/70‑1 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg < 1:246273/70‑1 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGGTCGGCAGCGCTGATTGTACCCATTGCgg < 1:3215925/70‑1 (MQ=255) tGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg < 1:1641517/69‑1 (MQ=255) tAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTc < 1:455093/71‑1 (MQ=255) tAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTc < 1:459038/71‑1 (MQ=255) tAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTc < 1:2706996/71‑1 (MQ=255) tAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTc < 1:2546234/71‑1 (MQ=255) tAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTc < 1:2542347/71‑1 (MQ=255) tAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTc < 1:1343769/71‑1 (MQ=255) aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:3009611/1‑58 (MQ=255) aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:492641/1‑58 (MQ=255) aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:674905/1‑58 (MQ=255) aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:377586/1‑58 (MQ=255) aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:752498/1‑58 (MQ=255) aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:2296830/1‑58 (MQ=255) aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:1743163/1‑58 (MQ=255) aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:3141944/1‑58 (MQ=255) aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:2078267/1‑58 (MQ=255) aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:2057594/1‑58 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg > 1:1906999/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg > 1:2375115/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg > 1:2436276/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg > 1:3357832/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg > 1:1880669/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg > 1:2990748/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTAGTCTg > 1:76227/1‑71 (MQ=255) ttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTAcc > 1:873994/1‑71 (MQ=255) ttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTAcc > 1:2549674/1‑71 (MQ=255) ttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTAcc > 1:2362762/1‑71 (MQ=255) tATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATTGGTACTTGGCTACCt > 1:1688498/1‑71 (MQ=255) tATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTAcc > 1:770962/1‑70 (MQ=255) tATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt > 1:1021653/1‑71 (MQ=255) tATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt > 1:2268078/1‑71 (MQ=255) tATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt > 1:1693531/1‑71 (MQ=255) tATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt > 1:1035111/1‑71 (MQ=255) tATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt > 1:1113158/1‑71 (MQ=255) tATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt > 1:1524554/1‑71 (MQ=255) tCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTAcc > 1:1667819/1‑61 (MQ=255) tCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCgg > 1:938306/1‑71 (MQ=255) tCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg > 1:1032406/1‑70 (MQ=255) cgcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCatt < 1:1891390/71‑1 (MQ=255) cgcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCatt < 1:2421807/71‑1 (MQ=255) gcTGATTGTACGCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCatt < 1:302580/70‑1 (MQ=255) gTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGcc < 1:553039/70‑1 (MQ=255) | GGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCC > W3110S.gb/47761‑47895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |