| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 515,934 | C→T | 49.4% | A43V (GCG→GTG) | ybbM → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 515,934 | 0 | C | T | 49.4% | 0.9 / 126.9 | 87 | A43V (GCG→GTG) | ybbM | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (16/28); new base T (22/21); total (38/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCCAG > W3110S.gb/515881‑515997 | ccATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatta < 1:2532352/71‑1 (MQ=255)ccATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatta < 1:147247/71‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCt < 1:2426561/63‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCt < 1:2430486/63‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCt < 1:2534481/63‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCt < 1:1847815/63‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCt < 1:1686344/63‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCt < 1:339819/63‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCt < 1:549246/63‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGat < 1:1063470/66‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGat < 1:2356829/66‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGat < 1:3245296/66‑1 (MQ=255) tAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGat < 1:1393253/66‑1 (MQ=255) aaGAAAAACTGGCGCTGGAGAATGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:3005508/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:2606576/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:2129658/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:3212561/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:1277896/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:1437993/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:250967/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:2602386/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:3070702/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:324925/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:2962809/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTc > 1:2543102/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTc > 1:603037/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTc > 1:2864569/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCGCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:2687163/1‑71 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc > 1:1910415/1‑70 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc > 1:3199827/1‑70 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc > 1:693927/1‑70 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc > 1:6275/1‑70 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc > 1:1819960/1‑70 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc > 1:1797193/1‑70 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc > 1:2841717/1‑70 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc > 1:1562064/1‑70 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc > 1:56557/1‑70 (MQ=255) aaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAAt > 1:2627854/1‑50 (MQ=255) cTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTAtgt < 1:2354852/71‑1 (MQ=255) cTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTAtgt < 1:293696/71‑1 (MQ=255) cTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTAtgt < 1:2350827/71‑1 (MQ=255) cTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTAtgt < 1:1653742/71‑1 (MQ=255) cTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCAGGCGAGCGATAATTCAGCTGATTATTGTCGGCTAtgt < 1:1914565/71‑1 (MQ=255) cgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTg < 1:796508/71‑1 (MQ=255) gcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTg < 1:345000/70‑1 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCg > 1:2040903/1‑50 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAttt > 1:3166299/1‑71 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAttt > 1:346584/1‑71 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAttt > 1:2691079/1‑71 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAttt > 1:611396/1‑71 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAttt > 1:739277/1‑71 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAttt > 1:1068754/1‑71 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAttt > 1:2006955/1‑71 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAttt > 1:149805/1‑71 (MQ=255) aGATATTATCTGGAGCGTCGGGCGAGTGATAATTCAGCTGa > 1:2297059/1‑41 (MQ=38) tGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTg < 1:2417738/69‑1 (MQ=255) tGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTg < 1:3295412/69‑1 (MQ=255) tGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTg < 1:420609/69‑1 (MQ=255) tGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTg < 1:1382516/69‑1 (MQ=255) ggAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTg < 1:2882460/68‑1 (MQ=255) gAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGTCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:1646993/70‑1 (MQ=255) gAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:1290657/70‑1 (MQ=255) gAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:631978/70‑1 (MQ=255) gAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:1075769/70‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:992220/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:1427499/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:1263200/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:1121979/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:96159/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:1776016/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:2627745/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:2574628/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:2271139/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:30546/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:2738646/67‑1 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:2565075/67‑1 (MQ=255) tCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:18212/65‑1 (MQ=255) tCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:265387/65‑1 (MQ=255) tCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:782149/65‑1 (MQ=255) tCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:2403325/65‑1 (MQ=255) tCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:2171543/65‑1 (MQ=255) tCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:1579686/65‑1 (MQ=255) tCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:1620668/65‑1 (MQ=255) gggCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgatga > 1:2564530/1‑65 (MQ=255) gggCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgatga > 1:142453/1‑65 (MQ=255) gggCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgatga > 1:897628/1‑65 (MQ=255) ggCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCCAg < 1:2428693/70‑1 (MQ=255) | CCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCCAG > W3110S.gb/515881‑515997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |