| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 539,323 | C→T | 18.7% | P318L (CCA→CTA) | allB → | allantoinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 539,323 | 0 | C | T | 18.7% | 66.8 / 10.1 | 32 | P318L (CCA→CTA) | allB | allantoinase |
| Reads supporting (aligned to +/- strand): ref base C (15/11); new base T (4/2); total (19/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01 | |||||||||||
AACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAG > W3110S.gb/539278‑539389 | aaCTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGt < 1:2972473/71‑1 (MQ=255)aaCTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGt < 1:2474722/71‑1 (MQ=255) tGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAg < 1:3173446/71‑1 (MQ=255) tGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAAt < 1:2662568/38‑1 (MQ=255) cTGGTTTCCGACCACTCTCTATGCCCGCCGGAAATGAAAGCCGGt < 1:1247815/45‑1 (MQ=255) cTGGTTTCCGACCACTCTCTATGCCCGCCGGAAATGAAAGCCGGt < 1:902767/45‑1 (MQ=255) ggTTTCCGACCACTCTCTATGCCCGCCGGAAATGAAAGCCGGTaa > 1:280361/1‑45 (MQ=255) ggTTTCCGACCACTCTCTATGCCCGCCGGAAATGAAAGCCGGTaa > 1:2731677/1‑45 (MQ=255) ggTTTCCGACCACTCTCTATGCCCGCCGGAAATGAAAGCCGGTaa > 1:2481204/1‑45 (MQ=255) ggTTTCCGACCACTCTCTATGCCCGCCGGAAATGAAAGCCGGTaa > 1:2141841/1‑45 (MQ=255) ggTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCa > 1:1974748/1‑58 (MQ=255) ggTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATg > 1:2146067/1‑60 (MQ=255) ggTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATg > 1:2053105/1‑60 (MQ=255) ggTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATg > 1:1072601/1‑60 (MQ=255) ggTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATg > 1:302969/1‑60 (MQ=255) ggTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGACGGTAACATCATGAAAGCATg > 1:868460/1‑60 (MQ=255) ccGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCgg < 1:650028/70‑1 (MQ=255) ccGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCgg < 1:756534/70‑1 (MQ=255) ccGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCgg < 1:409806/70‑1 (MQ=255) ccGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCgg < 1:3286901/70‑1 (MQ=255) ccGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCgg < 1:2467215/70‑1 (MQ=255) cGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCgg < 1:1657558/69‑1 (MQ=255) ctcCATGCCCGCCGGATATGAAAGCCGGTAACATCATGAAAGCa > 1:3174283/1‑44 (MQ=255) ctcCATGCCCGCCGGAAATGAAAGCCGGTAACATCATg > 1:2277493/1‑38 (MQ=255) ctcCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCa > 1:2428184/1‑44 (MQ=255) ctcCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCa > 1:683035/1‑44 (MQ=255) ctcCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCa > 1:943690/1‑44 (MQ=255) ctcCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCa > 1:577385/1‑44 (MQ=255) ctcCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCa > 1:1662782/1‑44 (MQ=255) ctcCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCa > 1:3171418/1‑44 (MQ=255) ctcCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCa > 1:2633393/1‑44 (MQ=255) tcCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAg < 1:498220/69‑1 (MQ=255) | AACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAG > W3110S.gb/539278‑539389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |