| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 98.4% | 103.8 / ‑6.4 | 30 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (1/28); minor base T (0/1); total (1/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGG > W3110S.gb/547653‑547759 | gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:2125147/71‑1 (MQ=255)gATGTGCAACCGGCTTCTTCACATATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:1142590/71‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGATGGCaaa < 1:3282561/40‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaa < 1:2362390/40‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaa < 1:687904/40‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaa < 1:541531/40‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaa < 1:2565697/40‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaa < 1:2375181/40‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaa < 1:202411/40‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaa < 1:1151262/40‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaa < 1:1336769/40‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaa < 1:1367119/40‑1 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt > 1:143530/1‑70 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg < 1:710124/70‑1 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg < 1:1003274/70‑1 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCGGGCAGGAGATGACCg < 1:378773/70‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:1931795/46‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:2240404/46‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:568830/46‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:3288396/46‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:2992784/46‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:3207377/46‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:1898207/53‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:2268177/53‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaa < 1:3250559/71‑1 (MQ=255) aaCGAGGGCAAAACACTTCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg < 1:1721938/71‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg < 1:2922330/71‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg < 1:2616966/71‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg < 1:1567423/71‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg < 1:2139862/71‑1 (MQ=255) | GATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGG > W3110S.gb/547653‑547759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |