Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	684,561	A→G	37.0%	L92L (TTA→CTA)	rihA ←	ribonucleoside hydrolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	684,561	0	A	G	37.0%	17.9 / 24.8	27	L92L (TTA→CTA)	rihA	ribonucleoside hydrolase 1
Reads supporting (aligned to +/- strand):  ref base A (15/2);  new base G (6/4);  total (21/6)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

AGCTCTACCGCCGTACAGTTTTGCGGTGCGAATGTCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACGCATT  >  W3110S.gb/684515‑684622                                               |                                                              aGCTCTACCGCCGTACAGTTTTGCGGTGCGAATGTCGGTTCCGGTaa                                                               >  1:30621/1‑47 (MQ=255) aGCTCTACCGCCGTACAGTTTTGCGGTGCGAATGTCGGTTCCGGTaa                                                               >  1:984773/1‑47 (MQ=255) aGCTCTACCGCCGTACAGTTTTGCGGTGCGAATGTCGGTTCCGGTaa                                                               >  1:1215788/1‑47 (MQ=255) aGCTCTACCGCCGTACAGTTTTGCGGTGCGAATGTCGGTTCCGGTaa                                                               >  1:2400175/1‑47 (MQ=255) aGCTCTACCGCCGTACAGTTTTGCGGTGCGAATGTCGGTTCCGGTaa                                                               >  1:2325653/1‑47 (MQ=255) aGCTCTACCGCCGTACAGTTTTGCGGTGCGAATGTCGGTTCCGGTaa                                                               >  1:1507445/1‑47 (MQ=255) aGCTCTACCGCCGTACAGTTTTGCGGTGCGAATGTCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTc                                       >  1:1504918/1‑71 (MQ=255)                     ttGCGGTGCGAATGTCGGTTCCGGTAGTGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGa                   >  1:818420/1‑71 (MQ=255)                     ttGCGGTGCGAATGTCGGTTCCGGTAGTGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGa                   >  1:445242/1‑71 (MQ=255)                     ttGCGGTGCGAATGTCGGTTCCGGTAGTGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGa                   >  1:362356/1‑71 (MQ=255)                     ttGCGGTGCGAATGTCGGTTCCGGTAGTGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGa                   >  1:2191776/1‑71 (MQ=255)                     ttGCGGTGCGAATGTCGGTTCCGGTAGTGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGa                   >  1:1458519/1‑71 (MQ=255)                     ttGCGGTGCGAATGTCGGTTCCGGTAGTGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGa                   >  1:3221718/1‑71 (MQ=255)                       gCGGTGCGAATGTCGGTTCCGGTAGTGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGAt                  <  1:1569812/70‑1 (MQ=255)                       gCGGTGCGAATGTCGGTTCCGGTAGTGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGAt                  <  1:2155210/70‑1 (MQ=255)                       gCGGTGCGAATGTCGGTTCCGGTAGTGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGAt                  <  1:2199413/70‑1 (MQ=255)                             cGAATGTCGGTTCCGGTAGTGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGAt                  <  1:323592/64‑1 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTg                                 >  1:352575/1‑43 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACg      >  1:3182262/1‑70 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACg      >  1:1452705/1‑70 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACg      >  1:366013/1‑70 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACg      >  1:1894326/1‑70 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACg      >  1:526512/1‑70 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACg      >  1:682781/1‑70 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACg      >  1:684942/1‑70 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACg      >  1:836101/1‑70 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACg      >  1:1264186/1‑70 (MQ=255)                                   tCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGACGTGCACATTGTCCGCGATAATCAACTCAc       >  1:1888454/1‑69 (MQ=255)                                     ggTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAAc           >  1:193382/1‑63 (MQ=255)                                     ggTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAAc           >  1:580258/1‑63 (MQ=255)                                     ggTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAAc           >  1:932454/1‑63 (MQ=255)                                      gTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACGCAtt  <  1:2349056/71‑1 (MQ=255)                                      gTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACGCAtt  <  1:3288395/71‑1 (MQ=255)                                               |                                                              AGCTCTACCGCCGTACAGTTTTGCGGTGCGAATGTCGGTTCCGGTAATGCCGGGCCGTCGAGACCGCTTTCGCCGTGCACATTGTCCGCGATAATCAACTCACGCATT  >  W3110S.gb/684515‑684622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑