| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 722,797 | C→T | 20.9% | T680T (ACG→ACA) | kdpD ← | fused sensory histidine kinase in two‑component regulatory system with KdpE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 722,797 | 0 | C | T | 20.9% | 88.2 / 18.4 | 43 | T680T (ACG→ACA) | kdpD | fused sensory histidine kinase in two‑component regulatory system with KdpE |
| Reads supporting (aligned to +/- strand): ref base C (17/17); new base T (6/3); total (23/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 | |||||||||||
ATCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGAAAGCGCCGCCAGCAGGGC > W3110S.gb/722745‑722841 | aTCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTaaa > 1:1261258/1‑71 (MQ=255)aTCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTaaa > 1:2307459/1‑71 (MQ=255)aTCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTaaa > 1:1423960/1‑71 (MQ=255)aTCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTaaa > 1:1491127/1‑71 (MQ=255)aTCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTaaa > 1:2622473/1‑71 (MQ=255)aTCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTaaa > 1:276374/1‑71 (MQ=255)aTCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTaaa > 1:2781481/1‑71 (MQ=255)aTCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTaaa > 1:1558025/1‑71 (MQ=255) tCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACTGTAAGCGGCGTGCGTAAAt < 1:3332505/71‑1 (MQ=255) tCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACTGTAAGCGGCGTGCGTAAAt < 1:1510463/71‑1 (MQ=255) tCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACTGTAAGCGGCGTGCGTAAAt < 1:929515/71‑1 (MQ=255) tCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAAt < 1:3284325/71‑1 (MQ=255) tCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAAt < 1:2055392/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:843784/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:39894/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:985863/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:3287708/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:2966084/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:2820867/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:867889/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:910363/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:2428868/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:2269618/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:1280377/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:2078278/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:1524205/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGa < 1:2367229/71‑1 (MQ=255) cTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCGAACAGCACCGTAAGCGGCGTGAGTAAATCATGCGa < 1:1521249/71‑1 (MQ=255) gATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACTGTAAGCGGCGTGCTTAAATCATGCGAAAGcgcc > 1:927674/1‑71 (MQ=255) gATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACTGTAAGCGGCGTGCGTAAATCATGCg > 1:260616/1‑63 (MQ=255) gATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACTGTAAGCGGCGTGCGTAAATCATGCGAAAgcg > 1:524662/1‑69 (MQ=255) gATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACTGTAAGCGGCGTGCGTAAATCATGCGAAAGcgcc > 1:157485/1‑71 (MQ=255) gATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACTGTAAGCGGCGTGCGTAAATCATGCGAAAGcgcc > 1:460168/1‑71 (MQ=255) gATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACTGTAAGCGGCGTGCGTAAATCATGCGAAAGcgcc > 1:640252/1‑71 (MQ=255) tAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGAAAGCGCCGCCAGCAggg > 1:1665018/1‑71 (MQ=255) tAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGAAAGCGCCGCCAGCAggg > 1:907118/1‑71 (MQ=255) tAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGAAAGCGCCGCCAGCAggg > 1:1952620/1‑71 (MQ=255) aaGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGAAAgcgc > 1:2097599/1‑59 (MQ=255) aaGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGAAAGCGCCGCCAGCAGGGc > 1:2040232/1‑71 (MQ=255) aaGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGAAAGCGCCGCCAGCAGGGc > 1:2043800/1‑71 (MQ=255) aaGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGAAAGCGCCGCCAGCAGGGc > 1:209412/1‑71 (MQ=255) aaGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGAAAGCGCCGCCAGCAGGGc > 1:1119311/1‑71 (MQ=255) aaGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGAGAAAGCGCCGCCAGCAGGGc > 1:1974938/1‑71 (MQ=255) | ATCCTTCGCTTGCCAGATCGAGCGTTAAGATTTCTGCCTGACCAAACAGCACCGTAAGCGGCGTGCGTAAATCATGCGAAAGCGCCGCCAGCAGGGC > W3110S.gb/722745‑722841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |