Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 70,434 C→T 100% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb70,4340CT95.7% 1155.1 / 26.6 374N16N (AAC→AATaraCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base C (0/16);  new base T (130/228);  total (130/244)
Fisher's exact test for biased strand distribution p-value = 1.86e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 374 total aligned reads displayed.

ATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGAC  >  W3110S.gb/70367‑70501
                                                                   |                                                                   
aTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGcc                                                                  <  1:2382867/71‑1 (MQ=255)
   gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCa                                                                >  1:260022/1‑70 (MQ=255)
   gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCAt                                                               >  1:216553/1‑71 (MQ=255)
   gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCAt                                                               >  1:351064/1‑71 (MQ=255)
      ggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCt                                                             <  1:234253/70‑1 (MQ=255)
      ggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCt                                                             <  1:2476671/70‑1 (MQ=255)
        aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                          <  1:93280/71‑1 (MQ=255)
        aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                          <  1:188910/71‑1 (MQ=255)
        aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                          <  1:2778893/71‑1 (MQ=255)
        aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                          <  1:685736/71‑1 (MQ=255)
          tATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                          <  1:1235206/69‑1 (MQ=255)
          tATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                          <  1:3219493/69‑1 (MQ=255)
           aTGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGc                                                       >  1:2003613/1‑71 (MQ=255)
              aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                    >  1:2403933/1‑71 (MQ=255)
              aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                    >  1:2497235/1‑71 (MQ=255)
               aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGt                                                   <  1:1637686/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCt                                                             <  1:3284116/59‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:484643/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:2210385/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:1045259/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:3186078/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:1959209/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:1342242/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:1907357/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:1873609/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:3178994/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:2540794/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:156123/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:1414948/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:2870250/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGtt                                                  >  1:1845340/1‑70 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTCGTGGCGGGttt                                                 <  1:75758/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATAACCTGCTGCCGGGATACTCGTTTAATGACCATCTGGTGGCGGGtt                                                  >  1:911495/1‑70 (MQ=255)
                  gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTa                                                <  1:1819453/71‑1 (MQ=255)
                  gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTa                                                <  1:2281302/71‑1 (MQ=255)
                   tATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTaa                                               >  1:1783919/1‑71 (MQ=255)
                   tATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTaa                                               >  1:3136149/1‑71 (MQ=255)
                       gCTGAAGCGCAAAATGATCCCCTGCTGCCTGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                           >  1:1661064/1‑71 (MQ=255)
                       gCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                           >  1:796344/1‑71 (MQ=255)
                       gCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                           >  1:2663882/1‑71 (MQ=255)
                       gCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                           <  1:973192/71‑1 (MQ=255)
                       gCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                           <  1:1465597/71‑1 (MQ=255)
                       gCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                           <  1:1103148/71‑1 (MQ=255)
                       gCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                           <  1:192381/71‑1 (MQ=255)
                        cTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCg                                          <  1:1023726/71‑1 (MQ=255)
                        cTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCg                                          <  1:2689221/71‑1 (MQ=255)
                        cTGAACCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCg                                          <  1:2823166/71‑1 (MQ=255)
                           aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                       <  1:1112971/71‑1 (MQ=255)
                           aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGTGTTTAACGCCGAtt                                       <  1:3313437/71‑1 (MQ=255)
                           aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                       <  1:3021984/71‑1 (MQ=255)
                             gcgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                     <  1:2717557/71‑1 (MQ=255)
                              cgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAg                                    <  1:659402/71‑1 (MQ=255)
                                cAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGc                                  <  1:1181864/71‑1 (MQ=255)
                                cAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGc                                  <  1:293379/71‑1 (MQ=255)
                                 aaaaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                 <  1:88407/71‑1 (MQ=255)
                                 aaaaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                 <  1:2641479/71‑1 (MQ=255)
                                    aTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAc                              >  1:1003771/1‑71 (MQ=255)
                                     tGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTaa                                               >  1:459253/1‑53 (MQ=255)
                                      gATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCt                                                             <  1:308466/38‑1 (MQ=255)
                                       aTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGt                           >  1:3249879/1‑71 (MQ=255)
                                       aTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGt                           <  1:92290/71‑1 (MQ=255)
                                       aTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGt                           <  1:3326353/71‑1 (MQ=255)
                                       aTCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGt                           <  1:2243418/71‑1 (MQ=255)
                                        tCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 <  1:2098767/48‑1 (MQ=255)
                                        tCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                          <  1:2909949/71‑1 (MQ=255)
                                        tCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                          <  1:1381482/71‑1 (MQ=255)
                                          cccTGCTGCCGTGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                 <  1:1752217/62‑1 (MQ=255)
                                            ctgctgCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACg                             <  1:1136265/64‑1 (MQ=255)
                                            ctgctgCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACg                             <  1:2511293/64‑1 (MQ=255)
                                              gctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtctc                     >  1:1212270/1‑70 (MQ=255)
                                              gctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtctc                     >  1:1323497/1‑70 (MQ=255)
                                               ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                           >  1:858719/1‑47 (MQ=255)
                                               ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                           >  1:3008153/1‑47 (MQ=255)
                                               ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                           >  1:1275854/1‑47 (MQ=255)
                                               ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCg                    <  1:2594257/70‑1 (MQ=255)
                                                tgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAt                  >  1:544741/1‑71 (MQ=255)
                                                  ccGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                    <  1:620167/35‑1 (MQ=255)
                                                     ggATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttttt             <  1:1542854/71‑1 (MQ=255)
                                                     ggATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttttt             <  1:516093/71‑1 (MQ=255)
                                                      gATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt            <  1:107197/71‑1 (MQ=255)
                                                      gATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt            <  1:243415/71‑1 (MQ=255)
                                                       aTACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt            <  1:1517701/70‑1 (MQ=255)
                                                       aTACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa           >  1:778258/1‑71 (MQ=255)
                                                        tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTAt          >  1:2132137/1‑71 (MQ=255)
                                                        tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTAt          >  1:398560/1‑71 (MQ=255)
                                                        tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTAt          >  1:2986336/1‑71 (MQ=255)
                                                        tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTAt          >  1:298048/1‑71 (MQ=255)
                                                        tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTAt          >  1:2049179/1‑71 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTAt          >  1:1493657/1‑69 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc         >  1:576977/1‑70 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc         >  1:1366403/1‑70 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc         >  1:2848121/1‑70 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc         >  1:158797/1‑70 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc         >  1:1676076/1‑70 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc         >  1:2457769/1‑70 (MQ=255)
                                                                tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa           >  1:2293549/1‑62 (MQ=255)
                                                                tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac  >  1:1300429/1‑71 (MQ=255)
                                                                tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac  >  1:3055061/1‑71 (MQ=255)
                                                                tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac  >  1:724856/1‑71 (MQ=255)
                                                                tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac  >  1:2617769/1‑71 (MQ=255)
                                                                   |                                                                   
ATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGAC  >  W3110S.gb/70367‑70501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: