| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 827,597 | C→T | 49.0% | P26P (CCG→CCA) | ybhS ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 827,597 | 0 | C | T | 49.0% | ‑0.6 / 72.2 | 51 | P26P (CCG→CCA) | ybhS | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (15/11); new base T (18/7); total (33/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCG > W3110S.gb/827531‑827664 | gTTAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGAt < 1:871257/71‑1 (MQ=255)gTTAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGAt < 1:1268226/71‑1 (MQ=255)gTTAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGAt < 1:1319836/71‑1 (MQ=255)gTTAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGAt < 1:710354/71‑1 (MQ=255)gTTAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGAt < 1:1564744/71‑1 (MQ=255) ttAATGTCGTAACCAAAAATAAACAGCAGTAGCAGGGGGATCACTACCGCAATCAGCCAGCTACTTGGAt < 1:2668272/70‑1 (MQ=255) ttAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGAt < 1:2926961/70‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGa < 1:2420056/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGa < 1:2588572/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGa < 1:213351/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGa < 1:26208/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGa < 1:3040674/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGa < 1:3108108/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGa < 1:1819208/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGa < 1:655729/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGa < 1:825814/71‑1 (MQ=255) tAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGtc < 1:3080105/71‑1 (MQ=255) tAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGtc < 1:1104803/71‑1 (MQ=255) gcagcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGca > 1:2224497/1‑70 (MQ=255) gcagcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGca > 1:89594/1‑70 (MQ=255) gcagcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGca > 1:134560/1‑70 (MQ=255) gcagcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGca > 1:1417297/1‑70 (MQ=255) gcagcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGca > 1:2096903/1‑70 (MQ=255) gcagcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGca > 1:3110600/1‑70 (MQ=255) gcagcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGca > 1:1893508/1‑70 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:2262483/1‑71 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:855953/1‑71 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:461452/1‑71 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:420417/1‑71 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:2899563/1‑71 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:2184559/1‑71 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:2282367/1‑71 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:2192617/1‑71 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:263690/1‑71 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:2718436/1‑71 (MQ=255) cagCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGa > 1:2921273/3‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:570740/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:1069540/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:2422673/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:790955/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:2568789/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:680203/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:1534083/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:1950298/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:479964/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:283457/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:2074125/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:419774/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:2690806/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:1933442/1‑71 (MQ=255) aCTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCAAAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:2093917/1‑71 (MQ=255) | GTTAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCG > W3110S.gb/827531‑827664 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |