| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 921,273 | G→A | 36.5% | D169N (GAT→AAT) | macB → | fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 921,273 | 0 | G | A | 36.5% | 47.9 / 49.9 | 52 | D169N (GAT→AAT) | macB | fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (11/22); new base A (10/9); total (21/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATC > W3110S.gb/921220‑921333 | aGCGCGTCAGCATCGCGCGGGCATTGATGATCGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:808907/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAGCCGGCGCa > 1:3250123/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCa > 1:493258/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCa > 1:441435/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGgcgaa > 1:22136/1‑69 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:1206659/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:372535/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:330625/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:2760856/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:2597939/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:2569770/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:2029744/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:1909386/1‑71 (MQ=255)aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGACGATGaaccaaa > 1:2074113/1‑63 (MQ=255) aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:920439/70‑1 (MQ=255) aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:1655771/70‑1 (MQ=255) aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:320987/70‑1 (MQ=255) aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:1785571/70‑1 (MQ=255) aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:2498139/70‑1 (MQ=255) aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:1507089/70‑1 (MQ=255) aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:1295787/70‑1 (MQ=255) gCATCGCGCGGGCATTGATGAACGGTGGTCAGGTATTTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:2503284/69‑1 (MQ=255) ggCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:1892482/59‑1 (MQ=255) atgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:3338033/71‑1 (MQ=255) atgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:3339676/71‑1 (MQ=255) atgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1356140/71‑1 (MQ=255) atgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1971398/71‑1 (MQ=255) atgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:2827319/71‑1 (MQ=255) tgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:2676628/70‑1 (MQ=255) tgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:2680510/70‑1 (MQ=255) gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1594235/56‑1 (MQ=255) gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:281810/56‑1 (MQ=255) gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:174696/56‑1 (MQ=255) gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:2435103/56‑1 (MQ=255) gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:2807976/56‑1 (MQ=255) aTTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATc < 1:2519317/70‑1 (MQ=255) ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1903782/52‑1 (MQ=255) ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:890416/52‑1 (MQ=255) ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1097281/52‑1 (MQ=255) ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:3310474/52‑1 (MQ=255) ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1823098/52‑1 (MQ=255) ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:187680/52‑1 (MQ=255) ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:445348/52‑1 (MQ=255) ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:58438/52‑1 (MQ=255) tacTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1252613/50‑1 (MQ=255) cctGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTgg > 1:2314852/3‑41 (MQ=255) cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:1787326/1‑45 (MQ=255) cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:873617/1‑45 (MQ=255) cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:3277843/1‑45 (MQ=255) cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:3248341/1‑45 (MQ=255) cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:3047346/1‑45 (MQ=255) cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:2677135/1‑45 (MQ=255) | AGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATC > W3110S.gb/921220‑921333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |