| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,020,867 | A→G | 68.6% | S159P (TCC→CCC) | sulA ← | SOS cell division inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,020,867 | 0 | A | G | 68.6% | 42.4 / 25.4 | 35 | S159P (TCC→CCC) | sulA | SOS cell division inhibitor |
| Reads supporting (aligned to +/- strand): ref base A (5/6); new base G (22/2); total (27/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
GGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTT > W3110S.gb/1020813‑1020927 | ggATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGc > 1:2984401/1‑70 (MQ=255) aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:1270929/1‑56 (MQ=255) aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:3222911/1‑56 (MQ=255) aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:2968204/1‑56 (MQ=255) aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:2757073/1‑56 (MQ=255) aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:735339/1‑56 (MQ=255) aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:2135137/1‑56 (MQ=255) aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:1822152/1‑56 (MQ=255) aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:928097/1‑56 (MQ=255) aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:788056/1‑56 (MQ=255) aTGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGa < 1:778636/70‑1 (MQ=255) aTGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGa < 1:2204706/70‑1 (MQ=255) agTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAAcacc < 1:1863626/71‑1 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAAcccc > 1:1824933/1‑70 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:2359054/1‑71 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:3004882/1‑71 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:1647052/1‑71 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:646407/1‑71 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:739519/1‑71 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:2148503/1‑71 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:2061252/1‑71 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:1900445/1‑71 (MQ=255) aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg > 1:772814/1‑71 (MQ=255) aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg > 1:1054178/1‑71 (MQ=255) aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg > 1:930950/1‑71 (MQ=255) aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg > 1:2871654/1‑71 (MQ=255) ttttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATaaa > 1:3149969/1‑60 (MQ=255) ttttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGt > 1:312655/1‑71 (MQ=255) ttttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGt > 1:1784598/1‑71 (MQ=255) ttttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGt > 1:1695531/1‑71 (MQ=255) ttAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGtt < 1:2097026/70‑1 (MQ=255) ttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGtt < 1:2030949/70‑1 (MQ=255) ttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGtt < 1:1389853/70‑1 (MQ=255) ttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCGTACCGGACGCATAATAAACCCCATAGCGtt < 1:2658110/70‑1 (MQ=255) cccGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata < 1:2636658/52‑1 (MQ=255) | GGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTT > W3110S.gb/1020813‑1020927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |