| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,032,491 | C→T | 63.3% | intergenic (‑357/‑70) | serT ← / → hyaA | tRNA‑Ser/hydrogenase 1, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,032,491 | 0 | C | T | 63.3% | 21.3 / 28.0 | 30 | intergenic (‑357/‑70) | serT/hyaA | tRNA‑Ser/hydrogenase 1, small subunit |
| Reads supporting (aligned to +/- strand): ref base C (7/4); new base T (13/6); total (20/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01 | |||||||||||
GTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAG > W3110S.gb/1032423‑1032546 | ttGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAg > 1:1635761/2‑71 (MQ=255)gTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAg > 1:2996735/1‑71 (MQ=255)gTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAg > 1:2985612/1‑71 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGcc > 1:86047/1‑70 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGcc > 1:3305825/1‑70 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGcc > 1:2502990/1‑70 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGAACGTCGCTTGcc > 1:1606507/1‑70 (MQ=255) tCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGa > 1:1405305/1‑48 (MQ=255) tCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGa > 1:903297/1‑48 (MQ=255) tCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGa > 1:705141/1‑48 (MQ=255) tCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGa > 1:416452/1‑48 (MQ=255) tCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGa > 1:3354631/1‑48 (MQ=255) tCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGa > 1:1229673/1‑48 (MQ=255) tCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGa > 1:1483248/1‑48 (MQ=255) tCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGa > 1:1982183/1‑48 (MQ=255) tCGATTTCAGCATCTCACGTGAAGCGATGGTTTGCCTAGCTATAGGGa > 1:1614000/1‑48 (MQ=37) aCGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTc > 1:2669692/1‑63 (MQ=255) aCGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTc > 1:2768312/1‑63 (MQ=255) aCGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTc > 1:1777630/1‑63 (MQ=255) aCGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTc > 1:216780/1‑63 (MQ=255) aaGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGc < 1:1807474/71‑1 (MQ=255) aaGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGc < 1:3187878/71‑1 (MQ=255) aaGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGc < 1:1923304/71‑1 (MQ=255) aaGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGc < 1:2791160/71‑1 (MQ=255) aaGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGc < 1:2454641/71‑1 (MQ=255) aGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGc < 1:2467005/70‑1 (MQ=255) tGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAaggag < 1:3317984/71‑1 (MQ=255) tGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAaggag < 1:1496557/71‑1 (MQ=255) tGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAaggag < 1:1426864/71‑1 (MQ=255) ccTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGGCGCAaggag < 1:2303121/64‑1 (MQ=255) | GTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAG > W3110S.gb/1032423‑1032546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |