| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,294,272 | C→T | 33.3% | L76L (CTG→CTA) | hns ← | global DNA‑binding transcriptional dual regulator H‑NS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,294,272 | 0 | C | T | 33.3% | 40.1 / 28.5 | 36 | L76L (CTG→CTA) | hns | global DNA‑binding transcriptional dual regulator H‑NS |
| Reads supporting (aligned to +/- strand): ref base C (12/12); new base T (9/3); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATTGCTGCAGTTTAC > W3110S.gb/1294204‑1294333 | tAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTcag > 1:993029/1‑71 (MQ=255) ttttGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgt < 1:2337650/71‑1 (MQ=255) ttttGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgt < 1:529371/71‑1 (MQ=255) ttttGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgt < 1:472421/71‑1 (MQ=255) ttttGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgt < 1:3188620/71‑1 (MQ=255) ttttGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgt < 1:2775021/71‑1 (MQ=255) ttttGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgt < 1:2771268/71‑1 (MQ=255) ttttGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgt < 1:1920087/71‑1 (MQ=255) ttttGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgt < 1:1677379/71‑1 (MQ=255) ttttGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcg < 1:337529/70‑1 (MQ=255) ttttGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcg < 1:2833046/70‑1 (MQ=255) cGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATAcc < 1:2016708/65‑1 (MQ=255) cGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATAcc < 1:2668801/65‑1 (MQ=255) tttAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATACCGt > 1:1871781/1‑65 (MQ=255) tttAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATACCGt > 1:1121495/1‑65 (MQ=255) tttAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATACCGt > 1:545420/1‑65 (MQ=255) tttAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATACCGt > 1:486504/1‑65 (MQ=255) tttAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATACCGt > 1:1354257/1‑65 (MQ=255) tttAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATACCGt > 1:2680242/1‑65 (MQ=255) tttAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATACCGt > 1:265240/1‑65 (MQ=255) tttAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATACCGt > 1:2170850/1‑65 (MQ=255) tttAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATACCGt > 1:195275/1‑65 (MQ=255) ttGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATAcc > 1:2378997/1‑56 (MQ=255) ttGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAt > 1:223554/1‑71 (MQ=255) ttGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAt > 1:2862521/1‑71 (MQ=255) ttGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAt > 1:1353147/1‑71 (MQ=255) ttGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAt > 1:1186560/1‑71 (MQ=255) tGGTGCCAGATTTAACGGCAGCAAGGCTATTTAGCAGTTCGTTCGGGTCAATAcc < 1:1435056/55‑1 (MQ=255) aGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTcgcg > 1:1258379/1‑69 (MQ=255) aGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGa > 1:548377/1‑70 (MQ=255) aGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGa > 1:2184370/1‑70 (MQ=255) ttAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATtg > 1:1672107/1‑71 (MQ=255) ttAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATtg > 1:1954998/1‑71 (MQ=255) ttAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATtg > 1:1413591/1‑71 (MQ=255) ttAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATtg > 1:1118177/1‑71 (MQ=255) aaGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATTGCTGCAGTTTAc < 1:3085578/71‑1 (MQ=255) aaGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATTGCTGCAGTTTAc < 1:608713/71‑1 (MQ=255) | TAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATTGCTGCAGTTTAC > W3110S.gb/1294204‑1294333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |