Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,633 G→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6330GT100.0% 103.6 / NA 34Q246Y (CAG→TAT) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/21);  total (13/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGA  >  W3110S.gb/1303566‑1303668
                                                                   |                                   
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                  <  1:2659418/71‑1 (MQ=255)
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                  <  1:2891890/71‑1 (MQ=255)
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                  <  1:2409289/71‑1 (MQ=255)
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                  <  1:171309/71‑1 (MQ=255)
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                  <  1:810717/71‑1 (MQ=255)
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                  <  1:217144/71‑1 (MQ=255)
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                  <  1:2220662/71‑1 (MQ=255)
  aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                >  1:569123/1‑71 (MQ=255)
  aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                >  1:3192547/1‑71 (MQ=255)
  aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                >  1:2189531/1‑71 (MQ=255)
  aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                >  1:1483640/1‑71 (MQ=255)
  aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                >  1:977315/1‑71 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGGGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:3025837/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:1237021/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:468913/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:834046/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:903076/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:2732688/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:2658724/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:255769/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:2255272/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:2009803/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                       <  1:1909049/71‑1 (MQ=255)
                           gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                  <  1:593846/44‑1 (MQ=38)
                                          acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg            <  1:2220283/51‑1 (MQ=255)
                                          acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg            <  1:1275726/51‑1 (MQ=255)
                                               gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTa                     >  1:979274/1‑37 (MQ=37)
                                               gcgAAAACCGTTATTAACTATGTAACCTATTTGACt                      >  1:2266994/1‑36 (MQ=25)
                                                     aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa  >  1:2442689/1‑50 (MQ=255)
                                                     aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa  >  1:650064/1‑50 (MQ=255)
                                                     aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa  >  1:821479/1‑50 (MQ=255)
                                                     aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa  >  1:1085095/1‑50 (MQ=255)
                                                     aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa  >  1:991171/1‑50 (MQ=255)
                                                     aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCAGAAGTTACCg   >  1:2562897/1‑49 (MQ=38)
                                                                   |                                   
TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGA  >  W3110S.gb/1303566‑1303668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑