| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,581 | C→T | 93.2% | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,581 | 0 | C | T | 93.2% | 1667.9 / 75.3 | 543 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (12/25); new base T (294/212); total (306/237) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
Only 100 of 575 total aligned reads displayed.
AAAGGTTATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGG > W3110S.gb/70513‑70648 | aaaGGTTATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:3141142/1‑71 (MQ=255) aaGGTTATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGc > 1:2741099/1‑71 (MQ=255) aGGTTATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGcc > 1:2230255/1‑71 (MQ=255) aGGTTATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGcc > 1:1421615/1‑71 (MQ=255) aGGTTATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGcc < 1:507549/71‑1 (MQ=255) ttATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc > 1:3052687/1‑71 (MQ=255) ttATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc > 1:708013/1‑71 (MQ=255) ttATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc > 1:1649121/1‑71 (MQ=255) ttATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc > 1:3262140/1‑71 (MQ=255) tataTTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc > 1:2343602/1‑71 (MQ=255) tataTTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc > 1:2832106/1‑71 (MQ=255) tataTTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc > 1:2954574/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc > 1:525121/1‑70 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCg > 1:170655/1‑71 (MQ=255) atTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGa > 1:308859/1‑66 (MQ=255) atTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGa > 1:367404/1‑66 (MQ=255) ttCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCg < 1:2420571/64‑1 (MQ=255) ctcAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:1878293/1‑71 (MQ=255) aaTCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat < 1:3331748/71‑1 (MQ=255) aTCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat < 1:1728409/70‑1 (MQ=255) aTCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtt > 1:143605/1‑71 (MQ=255) tctcACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtt > 1:2446333/1‑70 (MQ=255) tcACCATTCACGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg > 1:946767/1‑71 (MQ=255) aCCATTCGCGGTCAGGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:972673/1‑71 (MQ=255) aCCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:1808177/1‑70 (MQ=255) aCCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:3002758/70‑1 (MQ=255) aCCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt > 1:470321/1‑71 (MQ=255) aTTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGa > 1:1062780/1‑51 (MQ=255) aTTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt > 1:292005/1‑64 (MQ=255) aTTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGtt > 1:1158508/1‑71 (MQ=255) cgcgGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtt > 1:3230036/1‑60 (MQ=255) cgcgGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtt > 1:1964662/1‑60 (MQ=255) cgcgGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtt > 1:668547/1‑60 (MQ=255) cgcgGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTccc > 1:1767073/1‑71 (MQ=255) cgcgGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTccc > 1:2692916/1‑71 (MQ=255) cgcgGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTcc > 1:1135313/1‑70 (MQ=255) cgcgGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATATTGCTGTTccc > 1:2031374/1‑71 (MQ=255) cgGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTcc < 1:2526532/68‑1 (MQ=255) ggTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:2563308/71‑1 (MQ=255) ggTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:63036/71‑1 (MQ=255) cA‑GGGGGTTGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:208455/1‑70 (MQ=255) a‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:867745/1‑69 (MQ=255) gggggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgag < 1:1204503/71‑1 (MQ=255) gggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagag < 1:2632784/71‑1 (MQ=255) gggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga > 1:1097308/1‑68 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt < 1:2279095/71‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGata < 1:3187671/45‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg > 1:771107/1‑48 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt < 1:2065087/69‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:2076074/1‑71 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:2404338/1‑71 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:1940517/60‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgag < 1:3305321/63‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:101639/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:736120/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:1361654/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:1490206/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:1537018/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:1842980/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:2185700/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:2126482/71‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga > 1:2578454/1‑63 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga > 1:922202/1‑63 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:556580/1‑70 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:224793/1‑38 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:1676853/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:1260697/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:337315/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:1749914/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:2640843/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:1900793/56‑1 (MQ=255) aagtCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:1978448/4‑70 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:650948/1‑70 (MQ=255) aaTCAGGGACGAGAATTTGTTTCCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc > 1:436405/1‑64 (MQ=255) tCAGGTACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACg < 1:1589218/71‑1 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACg > 1:1239794/1‑71 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACg < 1:1321883/71‑1 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACg < 1:2876815/71‑1 (MQ=255) tCAGGGACGAGAATTTGTTTGACGACCTGGTGATATTTTGCAGtt > 1:835475/1‑45 (MQ=25) gggACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtc < 1:266335/71‑1 (MQ=255) gggACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtc < 1:1289655/71‑1 (MQ=255) gACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgt > 1:2795637/1‑71 (MQ=255) aCGAGAATTTGTTTGCCGCCCGGGTGATATTTTGCTGTTCCCGCCAGga > 1:1999647/1‑49 (MQ=39) aCGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:1122128/51‑1 (MQ=255) aCGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgtc < 1:906777/71‑1 (MQ=255) aCGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgt > 1:2594060/1‑70 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCa > 1:3025727/1‑71 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCa > 1:1386520/1‑71 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCa > 1:162127/1‑71 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCa < 1:3289892/71‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatc < 1:2941363/53‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:2550626/1‑69 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGa < 1:605374/71‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGa < 1:784477/71‑1 (MQ=255) ttGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:411075/1‑52 (MQ=255) ttGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:1927882/1‑52 (MQ=255) ttGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:2476861/1‑52 (MQ=255) ttGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:2311654/1‑52 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAgg > 1:2207286/1‑71 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAg > 1:1003123/1‑70 (MQ=255) | AAAGGTTATATTCTCAATCTCACCATTCGCGGTCA‑GGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGG > W3110S.gb/70513‑70648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |