| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,378,050 | G→A | 100% | A101A (GCG→GCA) | ycjR → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,378,050 | 0 | G | A | 79.0% | 33.5 / 5.3 | 19 | A101A (GCG→GCA) | ycjR | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (4/0); new base A (0/15); total (4/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCT > W3110S.gb/1377991‑1378100 | gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:2796529/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:816721/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:726125/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:706197/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:506946/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:3359981/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:1250525/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:2406385/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:2115492/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:1720714/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:1640797/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:1634456/68‑1 (MQ=255)gaacgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:1517937/68‑1 (MQ=255) aacgcATTCTTGAAGCGCTGGCAGAAGTTGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:2854038/68‑1 (MQ=255) acgcATTCTTGAAGCGCTGGCAGAAGTGGGCGGTAAAGGTATCGTCGTTCCGGCTGCATGGGGCATGtt < 1:2919041/68‑1 (MQ=255) ggCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCt > 1:2073260/1‑71 (MQ=255) ggCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCt > 1:3140332/1‑71 (MQ=255) ggCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCt > 1:1775320/1‑71 (MQ=255) ggCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCt > 1:762885/1‑71 (MQ=255) | GAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCT > W3110S.gb/1377991‑1378100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |