Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,692,839 G→A 100% G425D (GGT→GAT)  ydgA → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,692,8390GA100.0% 139.2 / NA 43G425D (GGT→GAT) ydgAconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/31);  total (12/31)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCA  >  W3110S.gb/1692772‑1692897
                                                                   |                                                          
gATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGAtt                                                          <  1:42383/70‑1 (MQ=255)
gATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGAtt                                                          <  1:595008/70‑1 (MQ=255)
gATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGAtt                                                          <  1:1561483/70‑1 (MQ=255)
gATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGAtt                                                          <  1:1757590/70‑1 (MQ=255)
gATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGAtt                                                          <  1:1958637/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:1101106/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:332202/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:3282926/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:3190414/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:3135557/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:631382/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:2491449/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:2486953/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:2445264/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:2365478/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:699106/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:168743/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:1316842/70‑1 (MQ=255)
                                     aGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:1228786/70‑1 (MQ=255)
                                      gTTTATGACTCAGGTAGCGATGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAg                   >  1:1086870/1‑71 (MQ=255)
                                      gTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAg                   >  1:21804/1‑71 (MQ=255)
                                      gTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAg                   >  1:1498019/1‑71 (MQ=255)
                                      gTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAg                   >  1:835754/1‑71 (MQ=255)
                                      gTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAg                   >  1:1437493/1‑71 (MQ=255)
                                      gTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAg                   >  1:92938/1‑71 (MQ=255)
                                      gTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAg                   >  1:1323793/1‑71 (MQ=255)
                                      gTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAg                   >  1:507761/1‑71 (MQ=255)
                                      gTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAg                   >  1:312010/1‑71 (MQ=255)
                                      gTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAg                   >  1:1127172/1‑71 (MQ=255)
                                                    tAGCGAAGCTGGAAGATTATCATGAAGATCAAGCGAAAAAACTGGCGAAACAGCa                     <  1:2930593/55‑1 (MQ=39)
                                                     aGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTgg                               >  1:967306/1‑44 (MQ=255)
                                                     aGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAg    >  1:259572/1‑71 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa  <  1:3323754/71‑1 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa  <  1:3327492/71‑1 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa  <  1:3123214/71‑1 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa  <  1:47954/71‑1 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa  <  1:2834337/71‑1 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa  <  1:2608675/71‑1 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa  <  1:803839/71‑1 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa  <  1:1467974/71‑1 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa  <  1:1386924/71‑1 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa  <  1:1292171/71‑1 (MQ=255)
                                                       cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAACGTGCATCAGCa  <  1:232224/71‑1 (MQ=255)
                                                                   |                                                          
GATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCA  >  W3110S.gb/1692772‑1692897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: