| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,793,630 | G→A | 25.4% | S35S (TCC→TCT) | ydiV ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,793,630 | 0 | G | A | 25.4% | 116.4 / 40.2 | 67 | S35S (TCC→TCT) | ydiV | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (39/11); new base A (5/12); total (44/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
ATGTTGCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAG > W3110S.gb/1793564‑1793696 | aTGTTTCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGgaga < 1:2268081/71‑1 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:2868713/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:1968092/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:2067674/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:3054431/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:1729527/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:564655/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:611403/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:770541/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:945044/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:2398223/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:2457685/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:247119/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:2490446/1‑68 (MQ=255) gCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGaaa > 1:2690156/1‑68 (MQ=255) ctcGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATggt < 1:442929/68‑2 (MQ=255) ctcGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATggg < 1:2222865/68‑1 (MQ=255) ggTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAAtt > 1:959067/1‑70 (MQ=255) ggTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAAtt > 1:3249934/1‑70 (MQ=255) ggTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAAtt > 1:2154532/1‑70 (MQ=255) ggTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAAtt > 1:3298418/1‑70 (MQ=255) ggTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAAtt > 1:1687142/1‑70 (MQ=255) ggTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACAATCTTCACTGGAGAAATGGGTAAtt > 1:418995/1‑70 (MQ=255) acacGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGc > 1:2997946/4‑64 (MQ=255) aCCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGt > 1:950746/1‑50 (MQ=255) aCCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAAt > 1:1881158/1‑53 (MQ=255) aCCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGc > 1:1885066/1‑64 (MQ=255) aCCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGc > 1:1478808/1‑64 (MQ=255) aCCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGc > 1:2782641/1‑64 (MQ=255) aCCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGc > 1:1183454/1‑64 (MQ=255) gACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACt > 1:1110977/1‑71 (MQ=255) gACTCGTCGGGATACGAACCGAACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGAc > 1:1202661/1‑64 (MQ=255) aTACGAACCGTACCATCTTCACTGGAGAAATGGGTAAtt > 1:946818/1‑39 (MQ=255) aTACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCaa > 1:1049525/1‑46 (MQ=255) aTACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCaa > 1:2185309/1‑46 (MQ=255) cGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTctct > 1:1114482/1‑71 (MQ=255) gAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:804784/71‑1 (MQ=255) gAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:3336052/71‑1 (MQ=255) gAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:326741/71‑1 (MQ=255) gAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:22098/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:2729317/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:1032026/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:1122655/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:555794/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:407262/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:3146651/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:2244287/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:2733167/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:2901584/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:2762767/71‑1 (MQ=255) gAACCGTACCATCTTCACTAGACAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:805145/71‑1 (MQ=255) aaCCGTACCATCTTCACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCtt < 1:1849583/70‑1 (MQ=255) ttCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTAt > 1:2804511/1‑60 (MQ=255) ttCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTAt > 1:2351159/1‑60 (MQ=255) ttCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTAt > 1:1222816/1‑60 (MQ=255) ttCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTAt > 1:529034/1‑60 (MQ=255) ttCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTAt > 1:1737546/1‑60 (MQ=255) ttCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGaaa > 1:2922224/1‑70 (MQ=255) cACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAg > 1:3116756/1‑71 (MQ=255) cACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAg > 1:150545/1‑71 (MQ=255) cACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAg > 1:787540/1‑71 (MQ=255) cACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAg > 1:1926140/1‑71 (MQ=255) cACTAGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAg > 1:2568778/1‑71 (MQ=255) tGGAGAAATGGGTAATTAACTCAACGCCGACAAgg < 1:1426384/35‑1 (MQ=255) tGGAGAAATGGGTAATTAACTCAACGCCGACAAgg < 1:2858526/35‑1 (MQ=255) tGGAGAAATGGGTAATTAACTCAACGCCGACAAgg < 1:2261597/35‑1 (MQ=255) tGGAGAAATGGGTAATTAACTCAACGCCGACAAgg < 1:1186771/35‑1 (MQ=255) | ATGTTGCTCCTCGGTAAGTTGCGCTATAACCCGACTCGTCGGGATACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAG > W3110S.gb/1793564‑1793696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |