| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,833,686 | G→A | 21.9% | P4L (CCA→CTA) | astC ← | succinylornithine transaminase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,833,686 | 0 | G | A | 21.9% | 80.8 / 18.6 | 41 | P4L (CCA→CTA) | astC | succinylornithine transaminase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (12/20); new base A (3/6); total (15/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GGTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAG > W3110S.gb/1833623‑1833743 | ggTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGTCTgaga < 1:916734/71‑1 (MQ=255)ggTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTgaga < 1:1226653/71‑1 (MQ=255)ggTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTgaga < 1:2747298/71‑1 (MQ=255)ggTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTgaga < 1:2264564/71‑1 (MQ=255)ggTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTgaga < 1:2058786/71‑1 (MQ=255)ggTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTgaga < 1:1672395/71‑1 (MQ=255)ggTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTgaga < 1:1561594/71‑1 (MQ=255)ggTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTgaga < 1:1224669/71‑1 (MQ=255)ggTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTgaga < 1:969113/71‑1 (MQ=255) gTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTgaga < 1:633014/70‑1 (MQ=255) ggAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTg > 1:2280771/1‑49 (MQ=255) ggAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATa > 1:80616/1‑60 (MQ=255) ggAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATa > 1:2483351/1‑60 (MQ=255) cATTCATCAAAGTTTTCACGCGTAATTAGCTGAGACATAGCGACCTCTACAGTAAATAATTCGt < 1:1825322/64‑1 (MQ=255) cATTCATCAAAGTTTTCACGCGTAATTAGCTGAGACATAGCGACCTCTACAGTAAATAATTCGt < 1:700408/64‑1 (MQ=255) cATTCATCAAAGTTTTCACGCGTAATTAGCTGAGACATAGCGACCTCTACAGTAAATAATTCGt < 1:534957/64‑1 (MQ=255) cATTCATCAAAGTTTTCACGCGTAATTAGCTGAGACATAGCGACCTCTACAGTAAATAATTCGt < 1:1766655/64‑1 (MQ=255) cATTCATCAAAGTTTTCACGCGTAATTAGCTGAGACATAGCGACCTCTACAGTAAATAATTCGt < 1:1751181/64‑1 (MQ=255) cATTCATCAAAGTTTTCACGCGTAATTAGCTGAGACATAGCGACCTCTACAGTAAATAATTCGt < 1:2988067/64‑1 (MQ=255) ttCATCAAAGTTTTCACGCGTAATTAGCTGAGACATAGCGCCCTCTACAGTAAATAATTCGtta > 1:3304188/1‑64 (MQ=255) ttCATCAAAGTTTTCACGCGTAATTAGCTGAGACATAGCGACCTCTACAGTAAATAATTCGtta > 1:951443/1‑64 (MQ=255) ttCATCAAAGTTTTCACGCGTAATTAGCTGAGACATAGCGACCTCTACAGTAAATAATTCGtta > 1:2952828/1‑64 (MQ=255) aaaGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:1793060/1‑70 (MQ=255) aaaGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:691652/1‑70 (MQ=255) ttCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAg > 1:989195/1‑71 (MQ=255) ttCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAg > 1:46318/1‑71 (MQ=255) ttCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAg > 1:717622/1‑71 (MQ=255) ttCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAg > 1:1473470/1‑71 (MQ=255) ttCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAg > 1:2512470/1‑71 (MQ=255) ttCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAg > 1:2408119/1‑71 (MQ=255) ttCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAg > 1:1982893/1‑71 (MQ=255) ttCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAg > 1:1984059/1‑71 (MQ=255) tAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt < 1:2527669/53‑1 (MQ=255) tAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt < 1:1586236/53‑1 (MQ=255) tAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt < 1:1820405/53‑1 (MQ=255) tAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt < 1:795752/53‑1 (MQ=255) tAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt < 1:1829983/53‑1 (MQ=255) tAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt < 1:3283534/53‑1 (MQ=255) tAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt < 1:3260450/53‑1 (MQ=255) tAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt < 1:3010769/53‑1 (MQ=255) tAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt < 1:2959597/53‑1 (MQ=255) tAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGt < 1:2622498/53‑1 (MQ=255) ggCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:218476/1‑51 (MQ=255) ggCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:325409/1‑51 (MQ=255) ggCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:1982728/1‑51 (MQ=255) ggCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:194038/1‑51 (MQ=255) ggCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:1915327/1‑51 (MQ=255) ggCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:3161538/1‑51 (MQ=255) ggCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:3013922/1‑51 (MQ=255) ggCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:1598212/1‑51 (MQ=255) ggCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaa > 1:1327218/1‑51 (MQ=255) | GGTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAATAAG > W3110S.gb/1833623‑1833743 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |