Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,887,874	T→C	68.6%	F106L (TTT→CTT)	yeaX →	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,887,874	0	T	C	68.6%	63.4 / 40.6	51	F106L (TTT→CTT)	yeaX	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (14/2);  new base C (11/24);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 2.42e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

CCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCC  >  W3110S.gb/1887810‑1887934                                                                 |                                                             ccGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTtcgc                                                        <  1:505071/71‑1 (MQ=255) ccGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTgcgc                                                        <  1:2015295/71‑1 (MQ=255) ccGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTgcgc                                                        <  1:1550269/71‑1 (MQ=255)       tttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAt                                                    <  1:2453186/69‑1 (MQ=255)       tttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAt                                                    <  1:2871298/69‑1 (MQ=255)       tttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAt                                                    <  1:486743/69‑1 (MQ=255)       tttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAt                                                    <  1:832139/69‑1 (MQ=255)               agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc                                           <  1:1159750/70‑1 (MQ=255)               agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc                                           <  1:442610/70‑1 (MQ=255)               agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc                                           <  1:1985682/70‑1 (MQ=255)               agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc                                           <  1:355485/70‑1 (MQ=255)               agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc                                           <  1:1049419/70‑1 (MQ=255)                    gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:971362/53‑1 (MQ=255)                    gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:1167384/53‑1 (MQ=255)                    gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:972942/53‑1 (MQ=255)                    gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:1902258/53‑1 (MQ=255)                    gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:3198472/53‑1 (MQ=255)                    gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:2873704/53‑1 (MQ=255)                    gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:98235/53‑1 (MQ=255)                    gTAAAAGTGGGCAATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:907418/53‑1 (MQ=255)                     tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:816745/52‑1 (MQ=255)                     tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:382507/52‑1 (MQ=255)                     tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt                                                       <  1:2762760/52‑1 (MQ=255)                             ggCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGtt                            <  1:1265548/71‑1 (MQ=255)                                   cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc                      >  1:3052798/1‑71 (MQ=255)                                              ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCATAAAGCATCTGTTTATCGCGGGCGGTATTg           >  1:3254766/1‑71 (MQ=255)                                              ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg           >  1:3174713/1‑71 (MQ=255)                                              ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg           >  1:710621/1‑71 (MQ=255)                                              ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg           >  1:8045/1‑71 (MQ=255)                                              ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg           >  1:2498686/1‑71 (MQ=255)                                              ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg           >  1:2000090/1‑71 (MQ=255)                                              ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg           >  1:3285196/1‑71 (MQ=255)                                              ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg           >  1:1489456/1‑71 (MQ=255)                                              ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg           >  1:1473543/1‑71 (MQ=255)                                              ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg           >  1:1049416/1‑71 (MQ=255)                                              ttCAACCCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTTTTTATCGCGGGCGGTATTg           >  1:905/1‑71 (MQ=255)                                                  aCGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt       <  1:1039895/71‑1 (MQ=255)                                                  aCGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt       <  1:1870212/71‑1 (MQ=255)                                                         ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc  >  1:1945439/1‑69 (MQ=255)                                                         ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc  >  1:879518/1‑69 (MQ=255)                                                         ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc  >  1:1243963/1‑69 (MQ=255)                                                         ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc  >  1:194538/1‑69 (MQ=255)                                                         ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc  >  1:2072966/1‑69 (MQ=255)                                                         ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc  >  1:3111262/1‑69 (MQ=255)                                                         ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc  >  1:3046155/1‑69 (MQ=255)                                                         ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc  >  1:2320563/1‑69 (MQ=255)                                                         ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc  >  1:2779430/1‑69 (MQ=255)                                                         ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAcc   >  1:2538899/1‑68 (MQ=255)                                                            aCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTa              >  1:1715607/1‑54 (MQ=255)                                                            aCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTa              >  1:2282227/1‑54 (MQ=255)                                                            aCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTa              >  1:1093129/1‑54 (MQ=255)                                                                 |                                                             CCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCC  >  W3110S.gb/1887810‑1887934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑