Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 70,740 T→G 94.4% G118G (GGT→GGG araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb70,7400TG94.4% 1425.8 / 34.5 406G118G (GGT→GGGaraCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (16/7);  new base G (221/162);  total (237/169)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 426 total aligned reads displayed.

TTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC  >  W3110S.gb/70673‑70806
                                                                   |                                                                  
tttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttc                                                                 >  1:15791/1‑71 (MQ=255)
  tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct                                                                <  1:1817350/70‑1 (MQ=255)
  tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct                                                                <  1:1495991/70‑1 (MQ=255)
  tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct                                                                <  1:2598764/70‑1 (MQ=255)
  tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct                                                                <  1:647159/70‑1 (MQ=255)
         gTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCg                                                        <  1:1825725/71‑1 (MQ=255)
                    tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa                                             >  1:1963560/1‑71 (MQ=255)
                      cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa                                             <  1:509545/69‑1 (MQ=255)
                      cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc                                            >  1:1442455/1‑70 (MQ=255)
                      cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc                                            >  1:3029453/1‑70 (MQ=255)
                      cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc                                            >  1:2694682/1‑70 (MQ=255)
                       tGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa                                             <  1:3159973/68‑1 (MQ=255)
                       tGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa                                             <  1:1940999/68‑1 (MQ=255)
                        ggCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAg                                                 >  1:1782381/1‑63 (MQ=255)
                        ggCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAg                                                 >  1:1369420/1‑63 (MQ=255)
                        ggCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAg                                         >  1:1861474/1‑71 (MQ=255)
                        ggCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAg                                         >  1:2284020/1‑71 (MQ=255)
                           aTGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGcct                                      <  1:2412312/71‑2 (MQ=255)
                            tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg                                               <  1:2608939/61‑1 (MQ=255)
                            tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg                                               <  1:256321/61‑1 (MQ=255)
                              aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCg                                                        >  1:2290593/1‑50 (MQ=255)
                              aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGa                                                   >  1:184558/1‑55 (MQ=255)
                              aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt                                   >  1:2964154/1‑71 (MQ=255)
                              aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt                                   >  1:1349263/1‑71 (MQ=255)
                              aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt                                   >  1:1311730/1‑71 (MQ=255)
                              aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt                                   >  1:3332688/1‑71 (MQ=255)
                              aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt                                   >  1:853325/1‑71 (MQ=255)
                              aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt                                   >  1:1987763/1‑71 (MQ=255)
                               aTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt                                  >  1:2458644/1‑71 (MQ=255)
                                tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt                                                               <  1:2723054/41‑1 (MQ=255)
                                tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt                                                               <  1:435702/41‑1 (MQ=255)
                                tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa                                                      >  1:2325397/1‑50 (MQ=255)
                                tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg                                                    <  1:2753699/52‑1 (MQ=255)
                                tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc                                            >  1:2246613/1‑60 (MQ=255)
                                tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc                                            <  1:755744/60‑1 (MQ=255)
                                tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc                                            >  1:1148339/1‑60 (MQ=255)
                                tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                 >  1:1561550/1‑71 (MQ=255)
                                tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt                                  >  1:2149393/1‑70 (MQ=255)
                                tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt                                  >  1:2273240/1‑70 (MQ=255)
                                  gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCTCCAGCCGCAttt                                 >  1:1042294/1‑69 (MQ=255)
                                  gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                 >  1:913948/1‑69 (MQ=255)
                                  gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                 >  1:3133168/1‑69 (MQ=255)
                                  gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                 >  1:898749/1‑69 (MQ=255)
                                  gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCCCCCGGATGAAGCGCACCAGCCGCAttt                                 >  1:3044217/1‑69 (MQ=255)
                                  gCTTAACTGGCCGTCAATATTTGCCAAAACGGGGTTCTTTCGCCCgg                                                       >  1:1118050/1‑47 (MQ=255)
                                       aCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCAGGATGAAgcg                                               <  1:2423266/50‑1 (MQ=255)
                                          ggCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                 >  1:2952656/1‑61 (MQ=255)
                                          ggCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                 >  1:2880533/1‑61 (MQ=255)
                                          ggCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                 >  1:1697229/1‑61 (MQ=255)
                                           gCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACGAGCCGCATTTCAGCGACCTg                       <  1:3256769/70‑1 (MQ=255)
                                           gCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg                       <  1:1672751/70‑1 (MQ=255)
                                           gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg                       <  1:394783/70‑1 (MQ=255)
                                           gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg                       <  1:2203074/70‑1 (MQ=255)
                                           gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg                       <  1:2360944/70‑1 (MQ=255)
                                           gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg                       <  1:2031211/70‑1 (MQ=255)
                                           gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg                       <  1:714659/70‑1 (MQ=255)
                                                 aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCt                        >  1:2679819/1‑63 (MQ=255)
                                                 aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt                     >  1:796391/1‑66 (MQ=255)
                                                 aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTggg                 >  1:205419/1‑70 (MQ=255)
                                                   tatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa               >  1:364047/1‑70 (MQ=255)
                                                    atTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaaa             >  1:2498291/1‑71 (MQ=255)
                                                    atTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaaa             >  1:2913605/1‑71 (MQ=255)
                                                    atTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaaa             >  1:1378104/1‑71 (MQ=255)
                                                    atTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaaa             >  1:1925687/1‑71 (MQ=255)
                                                     tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc                                            >  1:817945/1‑39 (MQ=255)
                                                     tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc                                            >  1:2988021/1‑39 (MQ=255)
                                                     tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                                     >  1:2855745/1‑46 (MQ=255)
                                                     tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaaa             >  1:3305347/1‑70 (MQ=255)
                                                     tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt            >  1:340720/1‑71 (MQ=255)
                                                     tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt            >  1:1180797/1‑71 (MQ=255)
                                                     tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt            >  1:1906856/1‑71 (MQ=255)
                                                     tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt            >  1:866066/1‑71 (MQ=255)
                                                       tGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCa          >  1:707626/1‑71 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCACATCAtt        <  1:431980/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:1212433/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:587075/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:735199/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:953174/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:1233477/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:2628261/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:3068695/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:2644281/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:1628750/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:2823139/71‑1 (MQ=255)
                                                         ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:1534403/71‑1 (MQ=255)
                                                          cAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt        <  1:2374791/70‑1 (MQ=255)
                                                            aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc                          <  1:2784109/50‑1 (MQ=255)
                                                            aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc                          <  1:561753/50‑1 (MQ=255)
                                                            aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc                          <  1:542472/50‑1 (MQ=255)
                                                            aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc                          <  1:147882/50‑1 (MQ=255)
                                                            aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt            <  1:3194445/64‑1 (MQ=255)
                                                             tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa                               <  1:334645/44‑1 (MQ=255)
                                                             tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa                               <  1:1002572/44‑1 (MQ=255)
                                                             tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACg    >  1:476194/1‑71 (MQ=255)
                                                             tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACg    >  1:3217891/1‑71 (MQ=255)
                                                             tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTAAGCGACCTGTTTGGGCAAATCATTAAc     >  1:2082922/1‑70 (MQ=255)
                                                              aCGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt                                   >  1:2313653/1‑39 (MQ=255)
                                                               cGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc  >  1:1068735/1‑71 (MQ=255)
                                                               cGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc  >  1:2112574/1‑71 (MQ=255)
                                                               cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc  >  1:2482695/1‑71 (MQ=255)
                                                                   |                                                                  
TTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC  >  W3110S.gb/70673‑70806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: