Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,939,329	C→T	27.6%	intergenic (+94/‑34)	yebK → / → pykA	predicted DNA‑binding transcriptional regulator/pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,939,329	0	C	T	27.6%	42.5 / 16.9	29	intergenic (+94/‑34)	yebK/pykA	predicted DNA‑binding transcriptional regulator/pyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base C (10/11);  new base T (5/3);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

ATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGG  >  W3110S.gb/1939283‑1939374                                               |                                              aTTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACg                       <  1:253896/71‑1 (MQ=255)    tATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGTAACACCTGGTTGTTTCAGTCAACGGAg                    >  1:1121056/1‑71 (MQ=255)    tATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGTAACACCTGGTTGTTTCAGTCAACGGAg                    >  1:3015016/1‑71 (MQ=255)    tATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGTAACACCTGGTTGTTTCAGTCAACGGAg                    >  1:287324/1‑71 (MQ=255)    tATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGTAACACCTGGTTGTTTCAGTCAACGGAg                    >  1:1619623/1‑71 (MQ=255)    tATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGTAACACCTGGTTGTTTCAGTCAACGGAg                    >  1:173855/1‑71 (MQ=255)        gCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGTAACACCTGGTTGTTTCAGTCAACGGAGTAt                 <  1:830171/70‑1 (MQ=255)        gCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGTAACACCTGGTTGTTTCAGTCAACGGAGTAt                 <  1:374205/70‑1 (MQ=255)        gCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGTAACACCTGGTTGTTTCAGTCAACGGAGTAt                 <  1:2526938/70‑1 (MQ=255)         ccATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTa               <  1:1106730/71‑1 (MQ=255)         ccATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTa               <  1:60768/71‑1 (MQ=255)         ccATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTa               <  1:3335807/71‑1 (MQ=255)         ccATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTa               <  1:2792196/71‑1 (MQ=255)         ccATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTa               <  1:2021399/71‑1 (MQ=255)         ccATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTa               <  1:1607167/71‑1 (MQ=255)         ccATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTa               <  1:1195227/71‑1 (MQ=255)          cATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTa               <  1:520332/70‑1 (MQ=255)          cATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTAc              <  1:71914/71‑1 (MQ=255)              gcggcgTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACgg                      >  1:3289997/1‑59 (MQ=255)              gcggcgTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATg           >  1:110227/1‑70 (MQ=255)              gcggcgTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGt          >  1:1945104/1‑71 (MQ=255)              gcggcgTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGt          >  1:2867492/1‑71 (MQ=255)              gcggcgTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGt          >  1:1280870/1‑71 (MQ=255)              gcggcgTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGt          >  1:1130480/1‑71 (MQ=255)              gcggcgTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGt          >  1:706729/1‑71 (MQ=255)              gcggcgTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGt          >  1:950767/1‑71 (MQ=255)                 gcgTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACg                       <  1:2073915/55‑1 (MQ=255)                      aTTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAgg  >  1:1578922/1‑71 (MQ=255)                      aTTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAg   >  1:3163372/1‑70 (MQ=255)                                               |                                              ATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGG  >  W3110S.gb/1939283‑1939374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑