| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,995,272 | T→C | 48.5% | S524S (TCA→TCG) | uvrC ← | excinuclease UvrABC, endonuclease subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,995,272 | 0 | T | C | 48.5% | ‑6.6 / 45.8 | 33 | S524S (TCA→TCG) | uvrC | excinuclease UvrABC, endonuclease subunit |
| Reads supporting (aligned to +/- strand): ref base T (8/9); new base C (12/4); total (20/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01 | |||||||||||
CGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAC > W3110S.gb/1995214‑1995339 | cGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTggcca > 1:3210038/1‑68 (MQ=255) gCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCa < 1:1386767/71‑1 (MQ=255) gCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCa < 1:1446452/71‑1 (MQ=255) gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa > 1:3020480/1‑71 (MQ=255) gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa > 1:581381/1‑71 (MQ=255) gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa > 1:3146995/1‑71 (MQ=255) gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa > 1:3153002/1‑71 (MQ=255) gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa > 1:2438752/1‑71 (MQ=255) aTGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTaaa < 1:3329047/63‑1 (MQ=255) aTGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTaaa < 1:3073252/63‑1 (MQ=255) aTGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTaaa < 1:2652469/63‑1 (MQ=255) aTGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCAc > 1:262751/1‑71 (MQ=255) aTGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCAc > 1:2513114/1‑71 (MQ=255) aTGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCAc > 1:2408129/1‑71 (MQ=255) aTGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCAc > 1:1823942/1‑71 (MQ=255) aTGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCAc > 1:1345871/1‑71 (MQ=255) cgcgAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTaaa < 1:3215307/52‑1 (MQ=255) gcgAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCa > 1:2955823/1‑70 (MQ=255) gcgAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCa > 1:577281/1‑70 (MQ=255) atGCTGGATAACATGCAGCGCGGGCGAATCTTGCGGCAAACTAAATc > 1:2032114/1‑47 (MQ=39) atGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:2740619/1‑50 (MQ=255) atGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:2635264/1‑50 (MQ=255) atGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:2181252/1‑50 (MQ=255) atGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:625152/1‑50 (MQ=255) atGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:677590/1‑50 (MQ=255) atGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:902490/1‑50 (MQ=255) gATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGt < 1:3318286/69‑1 (MQ=255) gggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAc < 1:2898680/71‑1 (MQ=255) gggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAc < 1:245391/71‑1 (MQ=255) gggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAc < 1:3330424/71‑1 (MQ=255) gggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAc < 1:403999/71‑1 (MQ=255) gggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCCCAAAGAACAGCGTTTCCAGTCCAGCCTTAc < 1:298444/71‑1 (MQ=255) gggTGAATCTGGCGGCAAACTAAATCCATCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAc < 1:1321108/71‑1 (MQ=255) | CGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAC > W3110S.gb/1995214‑1995339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |