| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,077,980 | C→T | 41.7% | Y355Y (TAC→TAT) | yeeR → | predicted membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,077,980 | 0 | C | T | 41.7% | 8.7 / 24.9 | 24 | Y355Y (TAC→TAT) | yeeR | predicted membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (2/12); new base T (2/8); total (4/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTA > W3110S.gb/2077930‑2078039 | aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCga < 1:544579/71‑1 (MQ=255)aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCga < 1:2631904/71‑1 (MQ=255)aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCga < 1:1479726/71‑1 (MQ=255)aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCga < 1:1100852/71‑1 (MQ=255)aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCga < 1:1849627/71‑1 (MQ=255)aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCga < 1:2366836/71‑1 (MQ=255)aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCga < 1:2470495/71‑1 (MQ=255)aCAGAAGTACATCATGCCCCGGAAAATCATAACCCGGATCCGGAATGGTACTCCTCCTCTCTTTGTCGCga < 1:3175196/71‑1 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTTTCGCgaaacaga < 1:721805/66‑1 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:1101787/66‑1 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:3227797/66‑1 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:2486283/66‑1 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:2187492/66‑1 (MQ=255) catGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTAc > 1:1777201/1‑39 (MQ=255) catGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTAc > 1:29010/1‑39 (MQ=255) catGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTAc > 1:3354440/1‑39 (MQ=255) atGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACtg > 1:1203267/1‑71 (MQ=255) atGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACt > 1:1399385/1‑70 (MQ=255) gAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGa < 1:637401/71‑1 (MQ=255) gAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGa < 1:579054/71‑1 (MQ=255) gAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGa < 1:2507920/71‑1 (MQ=255) gAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGa < 1:1386051/71‑1 (MQ=255) aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:920204/53‑1 (MQ=255) tAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:124700/48‑1 (MQ=255) aGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:3247719/46‑1 (MQ=255) ccGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCGCTTa > 1:3269186/1‑71 (MQ=255) ccGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTa > 1:474913/1‑71 (MQ=255) | ACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTA > W3110S.gb/2077930‑2078039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |