| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,157,782 | T→C | 14.8% | L128S (TTG→TCG) | mdtB → | multidrug efflux system, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,157,782 | 0 | T | C | 14.8% | 62.9 / 4.8 | 27 | L128S (TTG→TCG) | mdtB | multidrug efflux system, subunit B |
| Reads supporting (aligned to +/- strand): ref base T (11/12); new base C (2/2); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
TTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCAT > W3110S.gb/2157721‑2157845 | ttACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa < 1:3244689/71‑1 (MQ=255)ttACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa < 1:2336729/71‑1 (MQ=255) tCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAAccc < 1:1310226/70‑1 (MQ=255) tCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAAccc < 1:546452/70‑1 (MQ=255) tCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAAccc < 1:544209/70‑1 (MQ=255) gTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGtt < 1:902213/63‑1 (MQ=255) gTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGtt < 1:1025896/63‑1 (MQ=255) gTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGtt < 1:573632/63‑1 (MQ=255) gTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGtt < 1:2764572/63‑1 (MQ=255) gTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGtt < 1:2675085/63‑1 (MQ=255) gTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGtt < 1:1618973/63‑1 (MQ=255) gCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAg > 1:2003414/1‑71 (MQ=255) gCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAg > 1:463751/1‑71 (MQ=255) ttAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTaa > 1:2218677/1‑40 (MQ=255) ttAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTaa > 1:624931/1‑40 (MQ=255) ttAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTaa > 1:1614971/1‑40 (MQ=255) ttAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTaa > 1:477613/1‑40 (MQ=255) ttAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTaa > 1:163075/1‑40 (MQ=255) ttAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTaa > 1:2834388/1‑40 (MQ=255) ttAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTaa > 1:2800156/1‑40 (MQ=255) ttAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTaa > 1:203289/1‑40 (MQ=255) ttAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTaa > 1:2222595/1‑40 (MQ=255) ttAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAg < 1:1242450/60‑1 (MQ=255) ttAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAg < 1:2200189/60‑1 (MQ=255) ccAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGa > 1:1241494/1‑61 (MQ=255) ccAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt > 1:1929306/1‑70 (MQ=255) aaCTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCAt < 1:3245414/71‑1 (MQ=255) | TTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCAT > W3110S.gb/2157721‑2157845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |