Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,160,543 C→T 42.6% F7F (TTC→TTT mdtC → multidrug efflux system, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,160,5430CT42.6% 19.7 / 54.5 47F7F (TTC→TTTmdtCmultidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base C (15/12);  new base T (10/10);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

GTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGG  >  W3110S.gb/2160480‑2160596
                                                               |                                                     
gtgGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTAcc                                                >  1:2770605/1‑71 (MQ=255)
gtgGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTAcc                                                >  1:2428301/1‑71 (MQ=255)
                  tGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGAttt                              >  1:822411/1‑71 (MQ=255)
                  tGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGAttt                              >  1:1347886/1‑71 (MQ=255)
                          aTGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCg                      >  1:1641376/1‑71 (MQ=255)
                           tGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGc                                      <  1:2941197/54‑1 (MQ=255)
                             aaGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCg                                               >  1:2746558/1‑43 (MQ=255)
                             aaGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGcga                                    >  1:1005887/1‑54 (MQ=255)
                             aaGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGcga                                    >  1:1117198/1‑54 (MQ=255)
                             aaGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGcga                                    >  1:2872154/1‑54 (MQ=255)
                                aggaggCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTTCTGTCGGTTGc                 <  1:2253540/70‑1 (MQ=255)
                                   aggCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCAtt             <  1:989268/71‑1 (MQ=255)
                                   aggCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCAtt             <  1:3156745/71‑1 (MQ=255)
                                   aggCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCAtt             <  1:2313104/71‑1 (MQ=255)
                                   aggCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCAtt             <  1:2226700/71‑1 (MQ=255)
                                   aggCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCAtt             <  1:1586309/71‑1 (MQ=255)
                                    ggCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCAtt             <  1:2773439/70‑1 (MQ=255)
                                     gCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTAc           <  1:2008603/71‑1 (MQ=255)
                                     gCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTAc           <  1:952892/71‑1 (MQ=255)
                                     gCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTAc           <  1:2953547/71‑1 (MQ=255)
                                     gCGTAAGTGAAGTTTTTTGCACTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTTCCATTAc           <  1:539903/71‑1 (MQ=255)
                                        tAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTAc           <  1:2444203/68‑1 (MQ=255)
                                        tAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTAc           <  1:2505590/68‑1 (MQ=255)
                                          aGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTAc           <  1:419131/66‑1 (MQ=255)
                                           gTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc                 <  1:1073992/59‑1 (MQ=255)
                                           gTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc                 <  1:1124809/59‑1 (MQ=255)
                                           gTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc                 <  1:688181/59‑1 (MQ=255)
                                           gTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc                 <  1:1778943/59‑1 (MQ=255)
                                           gTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc                 <  1:2262086/59‑1 (MQ=255)
                                           gTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc                 <  1:1639252/59‑1 (MQ=255)
                                           gTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc                 <  1:2398216/59‑1 (MQ=255)
                                             gAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCa               >  1:1126111/1‑59 (MQ=255)
                                             gAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCa               >  1:3312859/1‑59 (MQ=255)
                                             gAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCa               >  1:1235687/1‑59 (MQ=255)
                                             gAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCa               >  1:1300015/1‑59 (MQ=255)
                                             gAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCa               >  1:1498757/1‑59 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTa            >  1:2524349/1‑61 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCgg  >  1:2919033/1‑71 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCgg  >  1:1276668/1‑71 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCgg  >  1:2999240/1‑71 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCgg  >  1:2459040/1‑71 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCgg  >  1:2353964/1‑71 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCgg  >  1:794628/1‑71 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCgg  >  1:1750802/1‑71 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCgg  >  1:960323/1‑71 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCg   >  1:2298350/1‑70 (MQ=255)
                                              aaGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCg   >  1:2533617/1‑70 (MQ=255)
                                                               |                                                     
GTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGG  >  W3110S.gb/2160480‑2160596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: