| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,313,823 | T→C | 44.4% | E349G (GAA→GGA) | yojL ← | predicted thiamine biosynthesis lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,313,823 | 0 | T | C | 44.4% | 17.3 / 82.6 | 63 | E349G (GAA→GGA) | yojL | predicted thiamine biosynthesis lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (13/22); new base C (17/11); total (30/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01 | |||||||||||
TTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATCGCCTTCTTTGGTGA > W3110S.gb/2313754‑2313889 | ttAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:901366/71‑1 (MQ=255)ttAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:2673115/71‑1 (MQ=255)ttAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:2069943/71‑1 (MQ=255)ttAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:2575751/71‑1 (MQ=255)ttAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:2531107/71‑1 (MQ=255)ttAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:1604909/71‑1 (MQ=255)ttAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:570637/71‑1 (MQ=255)ttAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:2490370/71‑1 (MQ=255)ttAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:2455301/71‑1 (MQ=255) tAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:1099971/70‑1 (MQ=255) gATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTc < 1:2395344/66‑1 (MQ=255) aTAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGa > 1:470792/1‑70 (MQ=255) aTAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTTAc < 1:2665162/71‑1 (MQ=255) aTAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGAc < 1:2298571/71‑1 (MQ=255) aTAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGAc < 1:1624453/71‑1 (MQ=255) aTAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGAc < 1:2436535/71‑1 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCtttt > 1:2601497/1‑70 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCtttt > 1:243439/1‑70 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCtttt > 1:3103812/1‑70 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCtttt > 1:1745754/1‑70 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCtttt > 1:582907/1‑70 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCtttt > 1:809687/1‑70 (MQ=255) tgccGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTa > 1:1904227/3‑70 (MQ=255) gTCACCATCACGCAAAATCCATCAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtgt < 1:2316802/71‑1 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtgt < 1:2816816/71‑1 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtgt < 1:2820752/71‑1 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtgt < 1:3317332/71‑1 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtgt < 1:562793/71‑1 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtgt < 1:65695/71‑1 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtgt < 1:94773/71‑1 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtgt < 1:2401746/71‑1 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtgt < 1:2276332/71‑1 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtct < 1:2541482/71‑3 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:1379237/71‑1 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:1088455/71‑1 (MQ=255) ccATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtggtg < 1:1484881/71‑1 (MQ=255) ccATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtggtg < 1:1429613/71‑1 (MQ=255) ccATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtggtg < 1:2112165/71‑1 (MQ=255) ccATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtggtg < 1:2273958/71‑1 (MQ=255) ccATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtggtg < 1:2467308/71‑1 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaaa > 1:2163710/1‑61 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaaa > 1:2813384/1‑61 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaaa > 1:654687/1‑61 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaaa > 1:1443843/1‑61 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaa > 1:872914/1‑60 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:1107381/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:1109211/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:107429/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:1073937/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:530905/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:3024434/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:3186638/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:2416626/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:1995138/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:2616993/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:2404037/1‑70 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:2412721/1‑70 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACAt > 1:1977263/1‑71 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACAt > 1:2962598/1‑71 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACAt > 1:2078210/1‑71 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACAt > 1:2083787/1‑71 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACAt > 1:2576312/1‑71 (MQ=255) tAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtgt < 1:778071/36‑1 (MQ=255) tttcCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATCGCCTTCTTTGGTGa < 1:703709/70‑1 (MQ=255) | TTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATCGCCTTCTTTGGTGA > W3110S.gb/2313754‑2313889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |