| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,327,759 | T→C | 43.8% | R408R (CGT→CGC) | atoC → | fused response regulator of ato opeon, in two‑component system with AtoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,327,759 | 0 | T | C | 43.8% | 6.7 / 38.1 | 32 | R408R (CGT→CGC) | atoC | fused response regulator of ato opeon, in two‑component system with AtoS |
| Reads supporting (aligned to +/- strand): ref base T (7/11); new base C (4/10); total (11/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
ACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAAC > W3110S.gb/2327699‑2327817 | acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAag < 1:1250881/71‑1 (MQ=255)acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAag < 1:635313/71‑1 (MQ=255)acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAag < 1:1519103/71‑1 (MQ=255)acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAag < 1:1394244/71‑1 (MQ=255)acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAag < 1:573138/71‑1 (MQ=255)acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAag < 1:421564/71‑1 (MQ=255)acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAag < 1:3327315/71‑1 (MQ=255)acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAag < 1:2719999/71‑1 (MQ=255)acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAag < 1:1793319/71‑1 (MQ=255)acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAag < 1:2045789/71‑1 (MQ=255) tAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaa > 1:1302710/1‑70 (MQ=255) tAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaa > 1:489326/1‑70 (MQ=255) tAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaa > 1:2343487/1‑70 (MQ=255) tAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAAGAGGAAATTAAACGCGTCGaaa < 1:933939/71‑1 (MQ=255) tAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAAGAGGAAATTAAACGCGTCGaaa < 1:1751657/71‑1 (MQ=255) aTGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAagag > 1:1980675/1‑50 (MQ=255) aTGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAagag > 1:347551/1‑50 (MQ=255) aTGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAagag > 1:2854344/1‑50 (MQ=255) aTGCTGGCGAGGTAAAAAAAGCCACTGTCGGTGAGCGCAATTTAAAagag > 1:3319725/1‑50 (MQ=255) cTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGc < 1:1766578/71‑1 (MQ=255) cTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGc < 1:1482665/71‑1 (MQ=255) cTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGc < 1:3340588/71‑1 (MQ=255) cTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGc < 1:1674109/71‑1 (MQ=255) cTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGc < 1:3289583/71‑1 (MQ=255) cTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGc < 1:264769/71‑1 (MQ=255) tAAAAACAGCCCCTGTCGGTGAGCGCAATTTAAAag < 1:3337070/36‑1 (MQ=255) aaaaCAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGa < 1:3232515/71‑1 (MQ=255) aaaaCAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGa < 1:1310938/71‑1 (MQ=255) gTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaac > 1:2616325/1‑70 (MQ=255) gTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaac > 1:141201/1‑70 (MQ=255) gTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaac > 1:1361423/1‑70 (MQ=255) gTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATAATGGAAGTGCTGGaac > 1:1626565/1‑70 (MQ=255) | ACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAAC > W3110S.gb/2327699‑2327817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |