Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,342,743 G→A 42.9% L450L (CTG→TTG)  gyrA ← DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,342,7430GA42.9% 8.1 / 30.9 28L450L (CTG→TTG) gyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base G (6/10);  new base A (5/7);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

CAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGC  >  W3110S.gb/2342683‑2342783
                                                            |                                        
cAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGgt                                        <  1:2370281/63‑1 (MQ=255)
cAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGgt                                        <  1:1091746/63‑1 (MQ=255)
cAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGgt                                        <  1:1318926/63‑1 (MQ=255)
cAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGgt                                        <  1:238010/63‑1 (MQ=255)
cAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGgt                                        <  1:742673/63‑1 (MQ=255)
cAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGgt                                        <  1:617972/63‑1 (MQ=255)
cAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGgt                                        <  1:241818/63‑1 (MQ=255)
cAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGgt                                        <  1:2147492/63‑1 (MQ=255)
        gTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATcacg                        <  1:2665114/71‑1 (MQ=255)
             tttCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAgg                                         <  1:1289557/49‑1 (MQ=255)
             tttCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAgg                                         <  1:2384233/49‑1 (MQ=255)
             tttCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAgg                                         <  1:2116798/49‑1 (MQ=255)
             tttCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAgg                                         <  1:2425654/49‑1 (MQ=255)
             tttCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAgg                                         <  1:282453/49‑1 (MQ=255)
             tttCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAgg                                         <  1:2923973/49‑1 (MQ=255)
             tttCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAgg                                         <  1:806162/49‑1 (MQ=255)
             tttCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAgg                                         <  1:130463/49‑1 (MQ=255)
                           gATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGgt                                        <  1:1858609/36‑1 (MQ=255)
                              ccAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGc  >  1:2845935/1‑71 (MQ=255)
                              ccAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGc  >  1:2261430/1‑71 (MQ=255)
                              ccAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGc  >  1:2214546/1‑71 (MQ=255)
                              ccAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGc  >  1:2184082/1‑71 (MQ=255)
                              ccAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGc  >  1:1860116/1‑71 (MQ=255)
                              ccAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGc  >  1:1515882/1‑71 (MQ=255)
                                 gAATCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCa                             <  1:2307906/41‑1 (MQ=255)
                                 gAATCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCa                             <  1:2168071/41‑1 (MQ=255)
                                 gAATCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCa                             <  1:1801546/41‑1 (MQ=255)
                                 gAATCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCa                             <  1:1526700/41‑1 (MQ=255)
                                 gAATCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCa                             <  1:3011626/41‑1 (MQ=255)
                                 gAATCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCa                             <  1:606962/41‑1 (MQ=255)
                                 gAATCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCa                             <  1:945109/41‑1 (MQ=255)
                                   aTCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCATc                           >  1:2116754/1‑41 (MQ=255)
                                   aTCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCATc                           >  1:1502694/1‑41 (MQ=255)
                                   aTCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCATc                           >  1:1394154/1‑41 (MQ=255)
                                   aTCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCATc                           >  1:1386677/1‑41 (MQ=255)
                                   aTCGCCTGAGCTTGCTGTTCGGTCAAGTAGTACAGACCATc                           >  1:833247/1‑41 (MQ=255)
                                                            |                                        
CAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGC  >  W3110S.gb/2342683‑2342783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑