| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,350,543 | G→A | 35.8% | V337I (GTA→ATA) | nrdA → | ribonucleoside diphosphate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,350,543 | 0 | G | A | 35.8% | 51.9 / 49.7 | 53 | V337I (GTA→ATA) | nrdA | ribonucleoside diphosphate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (23/11); new base A (11/8); total (34/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGT > W3110S.gb/2350476‑2350609 | aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:1065548/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:648336/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:618788/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:3240181/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:3099931/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:3033023/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:2853616/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:1520467/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:2427502/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:2208521/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:2113075/1‑70 (MQ=255)aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:1740338/1‑70 (MQ=255)aaGCCTGATGGTGTTGAAAAACAAACGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:1375309/1‑70 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:99629/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:909768/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:196137/70‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:3244698/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:1876099/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:2054203/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:1313772/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:1313785/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:3025608/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGGACAAATCAACAAACTGATg < 1:1821087/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:1125077/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:525006/71‑1 (MQ=255) aaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:510650/70‑1 (MQ=255) aaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:386340/70‑1 (MQ=255) aaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:99244/70‑1 (MQ=255) aaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:3102732/70‑1 (MQ=255) aaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:3221348/70‑1 (MQ=255) acCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATAcc > 1:106846/1‑71 (MQ=255) acCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATAcc > 1:203543/1‑71 (MQ=255) ggtgTGGAAGGCAACCGCGTGAGTCATATGGACTACTGGATACAAATCAACAAACTGATg < 1:694110/60‑1 (MQ=255) cgcgTGCGTCATATGGACTACGGGGTACAAATCATCAAACTGATg < 1:780904/45‑1 (MQ=255) cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:860538/1‑71 (MQ=255) cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:3284816/1‑71 (MQ=255) cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:3201338/1‑71 (MQ=255) cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:1372461/1‑71 (MQ=255) cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:171940/1‑71 (MQ=255) cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:2413328/1‑71 (MQ=255) cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:1722846/1‑71 (MQ=255) cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:1736324/1‑71 (MQ=255) cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCAGTTCAGCCCGt > 1:2781667/1‑71 (MQ=255) cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGAGAAGATATCACCCTGTTCAGCCCg > 1:889038/1‑70 (MQ=255) cGGGATACAAATCAACAAACTGATGTATACCCGTTTGCTGAAAGGTGAAGa > 1:2446576/1‑51 (MQ=255) cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGa > 1:756845/1‑48 (MQ=255) cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:1161723/1‑51 (MQ=255) cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:3092838/1‑51 (MQ=255) cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:2966303/1‑51 (MQ=255) cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:2479814/1‑51 (MQ=255) cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:1096382/1‑51 (MQ=255) cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:881951/1‑51 (MQ=255) cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:1024731/1‑51 (MQ=255) | AAGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGT > W3110S.gb/2350476‑2350609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |