Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,428,249 A→G 11.1% I246T (ATT→ACT)  yfcI ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,428,2490AG11.1% 354.5 / 29.1 126I246T (ATT→ACT) yfcIconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (66/46);  new base G (2/12);  total (68/58)
Fisher's exact test for biased strand distribution p-value = 3.05e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 128 total aligned reads displayed.

CTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGAA  >  W3110S.gb/2428179‑2428312
                                                                      |                                                               
ctcCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCaa                                                                 >  1:2932820/1‑71 (MQ=255)
      aGCAATACGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGtc                                                            <  1:2990146/70‑1 (MQ=255)
             cGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGttt                                                    >  1:2702614/1‑71 (MQ=255)
             cGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGtt                                                     >  1:2453965/1‑70 (MQ=255)
             cGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGtt                                                     >  1:2653101/1‑70 (MQ=255)
             cGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGtt                                                     >  1:485912/1‑70 (MQ=255)
             cGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGtt                                                     >  1:2997294/1‑70 (MQ=255)
             cGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGtt                                                     >  1:1013495/1‑70 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCATCAGTGGTCATCAGTTTCTCCtt                                              <  1:1181133/71‑1 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCtt                                              <  1:950148/71‑1 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCtt                                              <  1:1166895/71‑1 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCtt                                              <  1:865813/71‑1 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCtt                                              <  1:2254836/71‑1 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCtt                                              <  1:2447315/71‑1 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCtt                                              <  1:1439681/71‑1 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCtt                                              <  1:1532812/71‑1 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCtt                                              <  1:3211820/71‑1 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCtt                                              >  1:275455/1‑71 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCtt                                              >  1:2034359/1‑71 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCtt                                              >  1:3093778/1‑71 (MQ=255)
                   gCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCtt                                              >  1:992456/1‑71 (MQ=255)
                          cGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCTTTTCTtgt                                       >  1:1266302/1‑71 (MQ=255)
                          cGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCTTTTCTtgt                                       >  1:1944107/1‑71 (MQ=255)
                                 gCCCTGCTTTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:537835/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTTTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:2302005/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:1119930/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:1036728/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:2154142/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:3325858/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:3347337/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:1050125/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:2029217/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:2461691/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:464124/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:1324962/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:841410/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:1673300/70‑1 (MQ=255)
                                 gCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg                                 <  1:2440704/70‑1 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGtt                             >  1:2483648/1‑64 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGtt                             >  1:2234103/1‑64 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGtt                             >  1:1105090/1‑64 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGtt                             >  1:397023/1‑64 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGtt                             >  1:587589/1‑64 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGtt                             >  1:921938/1‑64 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGtt                             >  1:1529313/1‑64 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGtt                             >  1:1523902/1‑64 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGtt                             >  1:1458868/1‑64 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGt                              >  1:2468148/1‑63 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGt                              >  1:2599048/1‑63 (MQ=255)
                                           gcgcCTTCTTCACGTAATCTGTCAGCAAAGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGtt                             >  1:45110/1‑64 (MQ=255)
                                            cgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      <  1:3350636/70‑1 (MQ=255)
                                            cgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      <  1:2949760/70‑1 (MQ=255)
                                            cgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      <  1:1405719/70‑1 (MQ=255)
                                            cgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      <  1:2686170/70‑1 (MQ=255)
                                                  cttcACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCAcc                >  1:3304168/1‑70 (MQ=255)
                                                  cttcACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCAcc                >  1:761960/1‑70 (MQ=255)
                                                  cttcACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCAcc                >  1:760946/1‑70 (MQ=255)
                                                  cttcACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCAcc                >  1:1687164/1‑70 (MQ=255)
                                                  cttcACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCAcc                >  1:2178420/1‑70 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:3039895/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:1011651/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:2395856/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:2083992/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:1934699/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:1841810/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:218616/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:1818809/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:2226346/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:1257356/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:1065489/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:2377922/70‑1 (MQ=255)
                                                      aCGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATa            <  1:2435272/70‑1 (MQ=255)
                                                              tGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCAc     >  1:1196632/1‑69 (MQ=255)
                                                              tGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACg    >  1:852065/1‑70 (MQ=255)
                                                              tGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACg    >  1:1523752/1‑70 (MQ=255)
                                                              tGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACg    >  1:639797/1‑70 (MQ=255)
                                                              tGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACg    >  1:1954645/1‑70 (MQ=255)
                                                              tGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACg    >  1:1010755/1‑70 (MQ=255)
                                                              tGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACg    >  1:3303090/1‑70 (MQ=255)
                                                              tGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACg    >  1:2176935/1‑70 (MQ=255)
                                                              tGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACg    >  1:2332434/1‑70 (MQ=255)
                                                              tGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACg    >  1:2295235/1‑70 (MQ=255)
                                                                tCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGaa  <  1:2330092/70‑1 (MQ=255)
                                                                tCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGaa  <  1:1822674/70‑1 (MQ=255)
                                                                tCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGaa  <  1:526021/70‑1 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:2230473/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:2683836/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:991073/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:2836912/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:1444068/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:808583/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:2280258/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:2163895/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:1718274/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:2202938/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:310427/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:1904059/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:3359609/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:2034617/1‑48 (MQ=255)
                                                                  aGCAATGGTCATCAGTATCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt                      >  1:1645872/1‑48 (MQ=255)
                                                                      |                                                               
CTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGAA  >  W3110S.gb/2428179‑2428312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: