| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,462,408 | C→T | 18.3% | intergenic (‑150/+53) | sixA ← / ← yfcX | phosphohistidine phosphatase/fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,462,408 | 0 | C | T | 18.3% | 161.0 / 27.8 | 71 | intergenic (‑150/+53) | sixA/yfcX | phosphohistidine phosphatase/fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex |
| Reads supporting (aligned to +/- strand): ref base C (24/34); new base T (9/4); total (33/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTA > W3110S.gb/2462340‑2462463 | cTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt < 1:1016384/71‑1 (MQ=255)cTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt < 1:2188292/71‑1 (MQ=255)cTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt < 1:2749450/71‑1 (MQ=255)cTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt < 1:2796068/71‑1 (MQ=255)cTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt < 1:2829497/71‑1 (MQ=255)cTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt < 1:748384/71‑1 (MQ=255)cTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt < 1:934726/71‑1 (MQ=255) tCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt < 1:1115998/70‑1 (MQ=255) tCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt < 1:376217/70‑1 (MQ=255) tCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt < 1:309818/70‑1 (MQ=255) tCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt < 1:3095118/70‑1 (MQ=255) tCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt < 1:2788417/70‑1 (MQ=255) tCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt < 1:969893/70‑1 (MQ=255) aTTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt < 1:28517/67‑1 (MQ=255) cACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCaa < 1:563261/70‑1 (MQ=255) cACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCaa < 1:3272551/70‑1 (MQ=255) cACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCaa < 1:318638/70‑1 (MQ=255) cACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCaa < 1:3158186/70‑1 (MQ=255) cACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCaa < 1:779405/70‑1 (MQ=255) cACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCaa < 1:2486678/70‑1 (MQ=255) cACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCaa < 1:1034039/70‑1 (MQ=255) cACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCaa < 1:1643345/70‑1 (MQ=255) aCCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGATCaa < 1:3220071/69‑1 (MQ=255) gAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt > 1:2561967/1‑56 (MQ=255) gAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt > 1:3184604/1‑56 (MQ=255) gAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTg > 1:1930894/1‑57 (MQ=255) gAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTg > 1:2854618/1‑57 (MQ=255) gAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTg > 1:259983/1‑57 (MQ=255) gCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCtt > 1:1072492/1‑71 (MQ=255) gCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCtt > 1:1940156/1‑71 (MQ=255) gCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCtt > 1:748974/1‑71 (MQ=255) gCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCtt > 1:1113874/1‑71 (MQ=255) gCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCtt > 1:2588890/1‑71 (MQ=255) gCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCtt > 1:66382/1‑71 (MQ=255) gCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCtt > 1:1125085/1‑71 (MQ=255) gCGTAAGTATAGT‑AATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCtt > 1:1144266/1‑70 (MQ=255) tataGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa < 1:2223314/56‑1 (MQ=255) tataGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa < 1:130868/56‑1 (MQ=255) tataGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa < 1:649366/56‑1 (MQ=255) tataGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa < 1:2374581/56‑1 (MQ=255) taGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata > 1:825809/1‑56 (MQ=255) taGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata > 1:2786004/1‑56 (MQ=255) taGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata > 1:2923653/1‑56 (MQ=255) taGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata > 1:215456/1‑56 (MQ=255) taGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata > 1:1099676/1‑56 (MQ=255) taGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata > 1:3356164/1‑56 (MQ=255) taGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata > 1:1354592/1‑56 (MQ=255) taGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata > 1:2163111/1‑56 (MQ=255) taGTCAATATGCGTTTATATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata > 1:1331251/1‑56 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:660019/70‑1 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:2381404/70‑1 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:250459/70‑1 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:2613056/70‑1 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:907359/70‑1 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:667251/70‑1 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt > 1:2653003/1‑70 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:165023/70‑1 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:688007/70‑1 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTa > 1:2352852/1‑71 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTa > 1:3110081/1‑71 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTa > 1:3064215/1‑71 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTa > 1:2928485/1‑71 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTa > 1:2885591/1‑71 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTa > 1:1878276/1‑71 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTa > 1:2058805/1‑71 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTa > 1:2621909/1‑71 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTa > 1:2604917/1‑71 (MQ=255) gTCAATATGCGTTTACATTTTGACCAACACTCCGCAATTCAGCGCGGATTCATATAGca > 1:726333/1‑58 (MQ=255) gTCAATATGCGATTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:1046414/70‑1 (MQ=255) tCAATATGCGTTTACATTTTGACCAACACTCCGCCTTTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:872419/69‑1 (MQ=255) gTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt < 1:3235384/60‑1 (MQ=255) | CTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTA > W3110S.gb/2462340‑2462463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |