| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,508,088 | C→A | 17.1% | G615V (GGC→GTC) | ypdD ← | fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,508,088 | 0 | C | A | 17.1% | 348.4 / 38.4 | 121 | G615V (GGC→GTC) | ypdD | fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component |
| Reads supporting (aligned to +/- strand): ref base C (24/76); new base A (4/17); total (28/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 123 total aligned reads displayed.
ACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAA > W3110S.gb/2508027‑2508152 | actcatactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:2747362/1‑70 (MQ=255)actcatactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:2144684/1‑70 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCTGCAGATAACGGCTTTCACCGCCCAGTTCAcc > 1:1570243/1‑69 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCAcc > 1:2649249/1‑69 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCAcc > 1:2824943/1‑69 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCAcc > 1:2825963/1‑69 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCAcc > 1:2909395/1‑69 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCAcc > 1:143154/1‑69 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCAcc > 1:2684777/1‑69 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCAcc > 1:199059/1‑69 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCAcc > 1:1042245/1‑69 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCGCCGccc > 1:673768/1‑60 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:2503670/1‑66 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:2096504/1‑66 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:1187129/1‑66 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:1404362/1‑66 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:21586/1‑66 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:188154/1‑66 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:157338/1‑66 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCa < 1:1462626/71‑1 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCa < 1:2438424/71‑1 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCa < 1:1076396/71‑1 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCa < 1:2296306/71‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:965477/51‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:1830791/51‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:2274050/51‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:1700611/51‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:1742915/51‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:3170466/51‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:830121/51‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:785138/51‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:738175/51‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:1217115/51‑1 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAg < 1:1229113/51‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:1011335/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:2473564/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:694279/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:2572299/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:632649/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:2595386/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:49556/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:333701/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:2915766/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:1002921/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:1262806/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:1909401/50‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:1960258/50‑1 (MQ=255) ccAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:1510333/49‑1 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCAtt < 1:2559216/71‑1 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGtt > 1:1858484/1‑50 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGtt > 1:2639680/1‑50 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGtt > 1:3007683/1‑50 (MQ=255) ggCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTg > 1:1672056/1‑71 (MQ=255) ggCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTg > 1:2545966/1‑71 (MQ=255) ggCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTg > 1:2179852/1‑71 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:2685667/71‑1 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:3216898/71‑1 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:1256756/71‑1 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:2375206/71‑1 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:1601148/71‑1 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCAAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:841567/71‑1 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg < 1:2596710/39‑1 (MQ=255) ccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGc < 1:1059601/46‑1 (MQ=255) ccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGc < 1:1994821/46‑1 (MQ=255) ccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGc < 1:1988368/46‑1 (MQ=255) ccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGc < 1:246944/46‑1 (MQ=255) ccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGc < 1:1172118/46‑1 (MQ=255) ggCAGATAACGGCTTTCACCGCCCAGTTCACCGCTAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGt < 1:2542177/71‑1 (MQ=255) ggCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGt < 1:3157772/71‑1 (MQ=255) ggCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGt < 1:390227/71‑1 (MQ=255) gCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGt < 1:2324741/70‑1 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:1143231/71‑1 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:945690/71‑1 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:3184749/71‑1 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:303894/71‑1 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:2345356/71‑1 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:2154225/71‑1 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:2492551/71‑1 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:1726059/71‑1 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:2723060/71‑1 (MQ=255) gATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:3135515/70‑1 (MQ=255) gATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc < 1:3287149/70‑1 (MQ=255) aTAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTa < 1:555532/67‑1 (MQ=255) aTAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTa < 1:904625/67‑1 (MQ=255) aTAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTa < 1:2031484/67‑1 (MQ=255) aTAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTa < 1:3164834/67‑1 (MQ=255) aTAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTa < 1:344034/67‑1 (MQ=255) aTAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTa < 1:3089519/67‑1 (MQ=255) gCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCa < 1:1293320/36‑1 (MQ=255) gCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCa < 1:1750815/36‑1 (MQ=255) gCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCa < 1:1386134/36‑1 (MQ=255) gCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCa < 1:3266467/36‑1 (MQ=255) gCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCa < 1:2233316/36‑1 (MQ=255) gCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCa < 1:99189/36‑1 (MQ=255) cTTTCACCGCCCAGTTCACCGCAAATGCCTACCCa < 1:1268398/35‑1 (MQ=255) cACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCaa < 1:495365/70‑1 (MQ=255) cACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCaa < 1:2797972/70‑1 (MQ=255) cACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCaa < 1:391904/70‑1 (MQ=255) cACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCaa < 1:747282/70‑1 (MQ=255) cACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCaa < 1:2406080/70‑1 (MQ=255) | ACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAA > W3110S.gb/2508027‑2508152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |