| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,596,338 | T→C | 27.8% | intergenic (‑64/+149) | purC ← / ← nlpB | phosphoribosylaminoimidazole‑succinocarboxamide synthetase/lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,596,338 | 0 | T | C | 27.8% | 50.5 / 22.6 | 36 | intergenic (‑64/+149) | purC/nlpB | phosphoribosylaminoimidazole‑succinocarboxamide synthetase/lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (4/22); new base C (2/8); total (6/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
TGAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAAT > W3110S.gb/2596293‑2596400 | tgAATTAACGTTTTAAAATCTTTTGCTTTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGaa < 1:2922975/65‑1 (MQ=255)tgAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCTGATGCGGCGTGaa < 1:1263204/65‑1 (MQ=255)tgAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGaa < 1:288565/65‑1 (MQ=255)tgAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTCTTGTCGGATGCGGCGTGAACGc < 1:313440/68‑1 (MQ=255)tgAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTCTTGTCGGATGCGGCGTGAACGc < 1:2908270/68‑1 (MQ=255)tgAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTCTTGTCGGATGCGGCGTGAACGc < 1:255614/68‑1 (MQ=255)tgAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTCTTGTCGGATGCGGCGTGAACGc < 1:2190089/68‑1 (MQ=255)tgAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTCTTGTCGGATGCGGCGTGAACGc < 1:1850027/68‑1 (MQ=255)tgAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTCTTGTCGGATGCGGCGTGAACGc < 1:533379/68‑1 (MQ=255)tgAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTCTTGTCGGATGCGGCGTGAACGc < 1:1310979/68‑1 (MQ=255)tgAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTCTTGTCGGATGCGGCGTGAACGc < 1:1101775/68‑1 (MQ=255) aaTTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTAt > 1:706594/1‑71 (MQ=255) aaTTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTCTTGTCGGATGCGGCGTGAACGCCt > 1:2279543/1‑68 (MQ=255) aaTTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTCTTGTCGGATGCGGCGTGAACGCCt > 1:1013299/1‑68 (MQ=255) aaCGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCg < 1:474490/70‑1 (MQ=255) aaCGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCg < 1:389641/70‑1 (MQ=255) aaCGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCg < 1:1995639/70‑1 (MQ=255) aaCGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCg < 1:2140980/70‑1 (MQ=255) aaCGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCg < 1:2150290/70‑1 (MQ=255) aaCGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCg < 1:1609808/70‑1 (MQ=255) aaCGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCg < 1:1408062/70‑1 (MQ=255) aaCGTTTTAAAATCTTTTGCTGTCTGGTATGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCg < 1:3108800/70‑1 (MQ=255) aaCGTTTTAAAATCTTTAGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGAGGCGTGAACGCCTTATCCg < 1:805299/70‑1 (MQ=255) ttAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACg > 1:2626457/1‑71 (MQ=255) ttAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACg > 1:2389400/1‑71 (MQ=255) tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCg < 1:252261/70‑1 (MQ=255) tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCg < 1:179129/70‑1 (MQ=255) tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCg < 1:3291513/70‑1 (MQ=255) tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCg < 1:3339014/70‑1 (MQ=255) tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCg < 1:372399/70‑1 (MQ=255) tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCg < 1:1762812/70‑1 (MQ=255) tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCg < 1:1724035/70‑1 (MQ=255) tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCg < 1:1662102/70‑1 (MQ=255) tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCg < 1:1637169/70‑1 (MQ=255) tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCg < 1:1444909/70‑1 (MQ=255) ccGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAAt > 1:583686/1‑71 (MQ=255) | TGAATTAACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAAT > W3110S.gb/2596293‑2596400 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |