| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,667,558 | T→C | 35.5% | intergenic (‑6/‑130) | hcaR ← / → hcaE | DNA‑binding transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,667,558 | 0 | T | C | 35.5% | 24.7 / 27.8 | 31 | intergenic (‑6/‑130) | hcaR/hcaE | DNA‑binding transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base T (15/5); new base C (9/2); total (24/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
GCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAAC > W3110S.gb/2667521‑2667623 | gCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTgg > 1:999606/1‑69 (MQ=255)gCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTgg > 1:557622/1‑69 (MQ=255)gCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTgg > 1:499460/1‑69 (MQ=255)gCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTgg > 1:2902720/1‑69 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:1429895/1‑71 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:770986/1‑71 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:3173737/1‑71 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:3102082/1‑71 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:2978219/1‑71 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:283942/1‑71 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:2738448/1‑71 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:2149295/1‑71 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:2511653/1‑71 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:2064567/1‑71 (MQ=255) gCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTa > 1:1550961/1‑71 (MQ=255) tAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt < 1:2638686/71‑1 (MQ=255) tAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt < 1:2765212/71‑1 (MQ=255) tAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt < 1:2855405/71‑1 (MQ=255) tAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt < 1:300377/71‑1 (MQ=255) tAAATGCCGTAGTTCCATCACCTTACCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt < 1:150420/71‑1 (MQ=255) aaTGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTc > 1:2030463/1‑70 (MQ=255) tGCCGTAGTTCCATCACCTCCCCCTTGTTATGGAAAAAACGTCTCAGCTGGTAGTAATTAACTATTTcaca < 1:2634774/71‑1 (MQ=255) tGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTcaca < 1:1614241/71‑1 (MQ=255) aCCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt > 1:787229/1‑52 (MQ=255) aCCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac > 1:1050881/1‑70 (MQ=255) aCCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac > 1:853200/1‑70 (MQ=255) aCCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac > 1:163248/1‑70 (MQ=255) aCCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac > 1:1706014/1‑70 (MQ=255) aCCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac > 1:2017733/1‑70 (MQ=255) aCCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac > 1:2877533/1‑70 (MQ=255) aCCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac > 1:2771799/1‑70 (MQ=255) | GCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAAC > W3110S.gb/2667521‑2667623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |